Synthesis of high specific activity tritium labelled [2-3H]-adenosine-5'-triphosphates

Article abstract of DOI:10.1002/(SICI)1099-1344(199608)38:8<743::AID-JLCR884>3.0.CO;2-F

A procedure for high level tritium labelling at the C2-H position of adenosine 5'-triphosphate ([2-³H]-ATP, 1), based on the tritiodehalogenation reaction of 2-bromoadenosine 5'-triphosphate (2) has been elaborated. This precursor was prepared in a six-step synthesis from guanosine. The tritiodehalogenation of (2) for three hours over palladium oxide in phosphate buffer yielded tritium labelled ATP with high specific activity, in good chemical yield.