Structural studies of O-specific polysaccharide chains of the lipopolysaccharide from Yersinia enterocolitica serovar O:10

Article abstract of DOI:10.1016/0008-6215(94)00339-H

Lipopolysaccharide (LPS) was isolated from Yersinia enterocolitica serovars O:10 and O:10 KL and the structural pattern of O-specific sugar chains elucidated.The rhamman and L-xylulose (L-threo-pent-2-ulose) as constituents of the O-specific polysaccharide were obtained by autohydrolysis of the LPS.The rhamman was shown to be a linear, α-(1->3)-linked polysaccharide in the D configuration.L-Xylulose was purified using paper chromatography on a preparative scale and its structure was confirmed by 13C NMR spectroscopy.Using sugar and methylation analysis and 13C NMR s pectroscopy of the LPS and the rhamman, the structural features of the disaccharide repeating unit of the Y. enterocolitica O:10 O-specific polysaccharide were elucidated as:-3)-(1-> Keywords: Yersinia enterocolitica; Lipopolysaccharide; O-Specific polysaccharide; D-Rhamman; L-Xylulose (L-threo-Pent-2-ulose)