Bioscience, Biotechnology and Biochemistry p. 806 - 810 (1996)
Update date:2022-08-10
Topics:
Masuda, Masatoshi
Kishimoto, Daisuke
Kurihara, Norio
Bovine serum albumin (BSA) added to the reaction medium for the oxidative demethylation of 4-methoxyanisole and its 'di-CD3' isotopomer ([d6]methoxyanisole), when catalyzed by liver microsomes from untreated rats, decreased the K(m) values and increased the V(max)/K(m)(= V/K) values. The V(max) values were not markedly altered. The values for the deuterium isotope effect on V(max) and V/K for the reaction with this isotopomer were between 2.2 and 2.8, and that on K(m) was close to unity. The magnitude of the isotope effect was not significantly changed by adding BSA. The intramolecular isotope effect with [mono-CD3]4-methoxyanisole ([d3]methoxyanisole) in liver microsomes from untreated rats was between 10.3 and 10.8, which was not significantly changed by BSA. Liver microsomes from rats treated with phenobarbital resulted in the intramolecular isotope effect value in the absence of BSA being between 7.2 and 9.1, which was not significantly altered by BSA. Based on these data, the calculated apparent rate constant for the enzyme-substrate complex formation was markedly increased by up to about 1.9- and 3.5-fold by 1% and 2% of BSA added, respectively.
View MoreEASYBUYER Co.,LTD.( Chen-Chen )
Contact:+86-21-51816013/51816067
Address:1306,Bldg 8 Hi-Shanghai Center,No.950 Dalian Rd
Zhejiang Chemicals Import & Export Corporation
Contact:86-571-87043088
Address:No.37,Qingchun Road,Hangzhou,China
Contact:732.938.2777
Address:5012 Industrial Road Farmingdale, NJ 07727
Hebei Think-Do Environment Co., Ltd
website:http://www.thinkdo-environment.com
Contact:0311-86510809
Address:No 6, Shilian Middle Street, Circular Chemical Industry Park
Arshine Pharmaceutical Co., Limited
website:http://www.cnarshine.com
Contact:0731-88503671
Address:Room 1109.Block C3, Lugu Enterprise Plaza,No.27 Wenxuan Road,Changsha National Hi-Tech Industrial Development Zone,Hunan ,P.R.China
Doi:10.1016/j.bmc.2016.10.010
(2017)Doi:10.1080/00958972.2017.1387653
(2017)Doi:10.1016/0022-4596(78)90098-1
(1978)Doi:10.3390/molecules19056727
(2014)Doi:10.1080/00397910600602537
(2006)Doi:10.1016/0022-328X(89)87284-5
(1989)