STUDIES ON TRIFLUOROMETHYLPHOSPHONAMIDITE ANALOGUES AS BUILDING BLOCKS IN OLIGONUCLEOTIDE SYNTHESIS

Article abstract of DOI:10.1016/0040-4039(95)00186-G

The phosphorylating reagents CF3P(NMe2)Cl and CF3P(NEt2)Cl are used to phosphorylate the 3'-hydroxyl moiety of several protected 2'-deoxynucleosides yielding the corresponding nucleoside trifluoromethyl phosphonamidites.Their scope and limitations towards amidite activation are investigated but, however, their behavior is completely different to commonly used nucleoside phosphorus amidites.