Enzymatic synthesis of L-tagatose from galactitol with galactitol dehydrogenase from Rhodobacter sphaeroides D

Article abstract of DOI:10.1016/S0008-6215(97)00265-6

The rare sugar L-tagatose was prepared with an overall yield of 78% by enzymatic C-5 oxidation of galactitol with co-substrate regeneration, and purification by ligand exchange chromatography. The sugar was identified by HPLC, melting point determination, optical rotation, and NMR spectrometry.