Ring-A cleavage of 3-oxo-olean-12-en-28-oic acid by the fungus Chaetomium longirostre

Article abstract of DOI:10.1016/0031-9422(96)00266-X

3-Oxo-olean-12-en-28-oic acid was transformed by the filamentous fungus Chaetomium longirostre into 3,4-seco-olean-12-en-4-ol-3,28-dioic acid and the 21β-hydroxylated compound. A cell-free preparation derived from the fungus converted 3-oxo-olean 12-en-28-oic acid into 3,4-seco-olean-12-en-4-ol-3,28- dioic acid. The ring-A cleavage activity was detected in the soluble fraction of the cell-free preparation and showed a requirement for NADPH.