GIBBERELLINS A82 and A83 IN SEED OF LUPINUS ALBUS

Article abstract of DOI:10.1016/0031-9422(92)80325-9

Extracts from seeds of Lupinus albus at 14, 22, 35 and 52 days after anthesis were seperated into free gibberellins, ester conjugates and ether conjugates.Capillary GC-MS of the methylated and trimethylsilylated free gibberellin fractions showed the presence of the known gibberellins A₁, A₃, A₄, A₁₇, A₁₈, A₂₃ and A₄₃.In addition six new gibberellin-like compounds were detected that corresponded to the addition of the elements of water to gibberellins A₃, A₄, A₇, A₁₄, A₁₈ and A₃₄.Two of these components were identified by chemical syntheses as ent-3α,10β,17-trihydroxy-20-nor-16αHgibberellane-7,19-dioic acid 19,10-lactone and ent-3α,17-dihydroxy-16αHgibberellane-7,19-dioic acid, which are accorded the gibberellin numbers A₈₂ and A₈₃, respectively, ent-3α,10β,16β,17-tetrahydroxy-20-nor-16βHgibberellane-7,19-dioic acid 19,10-lactone was also identified by synthesis of the methyl ester.Similar analyses of the hydrolysed ether conjugate fractions showed the presence of the known gibberellins A₁, A₃, A₁₃, A₁₈ and A₄₃, the new gibberellin A₈₂ and the "hydrated" gibberellins A₁₈ and A₃₄; the 15-ene isomers of gibberellins A₁₃ and A₄₃ and the 16ξ17-epoxide of gibberellin A₁₈ were also identified as probable artefacts.In the hydrolysed ester conjugate fractions the new gibberellin A₈₂ and the "hydrated" gibberellin A₃₄ were detected.Gibberellin A₁₈ was by far the most abundant GA but quantitation of the GAs was not carried out.