CHENODEOXYCHOLIC ACID SYNTHESIS IN THE HAMSTER: A METABOLIC PATHWAY VIA 3β,7α-DIHYDROXY-5-CHOLEN-24-OIC ACID

Article abstract of DOI:10.1016/0039-128X(82)90078-2

The quantitative significance of the metabolism of 3β,7α-dihydroxy-5-cholen-24-oic acid to chenodeoxycholic acid was evaluated in the hamster.A precursor-product relationship was established in this species by the finding that intravenous administration to an animal previously given cholesterol-4-14C caused a significant reduction in the specific activity of chenodeoxycholic acid.Administration of 12.9 μmole of the precursor was followed by a 10-fold increase in chenodeoxycholic acid excretion although the predominant excretory pathway was via biliary excretion as a monosulfate.The data indicate that synthesis of bile acid from cholesterol via the intermediate 3β,7α-dihydroxy-5-cholen-24-oic acid can be a quantitatively important pathway.