Determination of Furosine as a Measure for Irreversibly Bound Glucose in Human Fibrinogen

Article abstract of DOI:10.1007/BF00809298

An analytical procedure is presented which enables for the determination of irreversibly, nonenzymatic glycation of fibrinogen isolated from human blood.The method is based on determination of furosine, which is formed during hydrolysis of Amadori-products.For validation of the worked out furosine assay, synthesis of N_α-formyl-N_ε-(desoxy-1-D-fructosyl-1)-L-lysine as a model substance and furosine as a calibration standard were necessary.Several hydrolysis experiments showed the extent of furosine formation from fructosyllysine and from human fibrinogen.The increase of fibrinogen glycation by incubation with D-glucose could also be confirmed according to the literature.Using well-known techniques for sampling, work up and in performing reduction and hydrolysis steps, quantitative determination of furosine by high-performance liquid chromatography is possible.By application of the analytical assay, the extent of glycation of human fibrinogen can be analyzed with good precision.Calibration is performed by means of a prepared furosine standard.The practical handling of the method is shown. - Keywords.Human fibrinogen; Furosine; Glycation; HPLC; N_α-Formyl-N_ε-(desoxy-1-D-fructosyl-1)-L-lysine.