Journal of Organic Chemistry p. 5790 - 5794 (1995)
Update date:2022-08-11
Topics:
Pirrung
Cao
Chen
In order to understand both the substrate specificity and active site topography of the ethylene-forming enzyme (EFE), a number of analogs of its substrate, 1-aminocyclopropanecarboxylic acid, have been prepared and studied as inhibitors. Because of the dependence of EFE activity on iron, hydroxamic acids, a functional group known to bind iron tightly, derived from several small carboxylic/amino acids were studied along with the parent amino acids. The activity of these materials was assayed in vitro against the purified EFE from apple fruit. The varying potency of the amino acid hydroxamates suggests that they do not act simply by binding to iron and removing it from the enzyme. The order of their potency was consistent with the idea that binding reflects both metal chelation and hydrophobic interactions in the active site. The most potent inhibitor, ACC-hydroxamate, has about 1 μM K(i).
View MoreShanghai Sinofluoro Scientific Co., Ltd
Contact:+86-21-64279360
Address:Room 1006,Building 3,#58 East Xinjian Road, Shanghai ,201100,China,
Shaanxi Mingqi Chemical Co., Ltd
website:http://mingqichem.lookchem.com/
Contact:+8618092428356
Address:Weiyang District
Shanghai Yuanye Bio-Technology Co., Ltd.
website:http://www.shyuanye.com
Contact:+86-21-61845781
Address:Building 6, No. 465, Changta Road,Songjiang District,Shanghai,China
Zhejiang Sanmei Chemical Industry Co., Ltd
Contact:86-579-87633213
Address:Huchu Industrial Zone, Qingnian Rd., Wuyi County, Zhejiang Prov., China.
Basilea Pharmaceutica China Ltd.
Contact:+86-513-82198075
Address:No.638 Xiushan Road(East),Haimen, Jiangsu 226100, P.R.China
Doi:10.1109/2943.838041
(1895)Doi:10.1007/BF00475579
(1988)Doi:10.1134/S0036024420050222
(2020)Doi:10.1016/S0277-5387(00)80116-3
(1993)Doi:10.1139/v93-061
(1993)Doi:10.1016/j.bioorg.2019.04.008
(2019)