W. Li et al. / Phytochemistry 70 (2009) 816–821
821
[M+Na]+. HRESIMS (positive) m/z 1245.5923 [M+Na]+ (calcd. for
C58H94O27Na, 1245.5880).
MeOH (95:5) as eluate to afford soyasapogenol E (2.8 mg), soyas-
apogenol A (2.4 mg), and echinocystic acid (2.4 mg), which showed
identical spectroscopic data with authentic samples. The aqueous
layers obtained above were concentrated under reduced pressure
to give sugar fractions. Identification of the monosaccharides pres-
ent in sugar fractions was carried out by the same procedure as in
4.7. Impatienoside D (10)
Amorphous solid, ½a D22
ꢀ
+12.0 (c 0.83, MeOH); IR (KBr) kmax:
3407, 2926, 1721, 1628, 1412, 1384, 1255, 1224 cmꢁ1. For 1H
NMR (500 MHz, pyridine-d5) and 13C NMR (125 MHz, pyridine-
d5) spectroscopic data, see Tables 1–4. ESIMS (positive) m/z 1275
[M+Na]+. HRESIMS (positive) m/z 1275.6033 [M+Na]+ (calcd. for
C59H96O28Na, 1275.5986).
literature (Li et al., 2007). tR (min): 11.30 (
-glucose), 10.57 ( -galactose), 7.55 ( -rhamnose), 6.28 (
nose), and 6.25 ( -xylose).
D
-glucuronic acid), 10.67
(D
D
L
L
-arabi-
D
4.12. Cytotoxic assays
4.8. Impatienoside E (12)
The human HL-60 myeloid leukemia, human stomach KATO-III
adenocarcinoma, and human lung A549 adenocarcinoma cell lines
assays were performed as the same procedures as in our previous
report (Chang et al., 2007).
Amorphous solid, ½a D22
ꢀ
+11.8 (c 1.00, MeOH); IR (KBr) kmax:
3416, 2926, 1735, 1628, 1408, 1383, 1251 cmꢁ1. For 1H NMR
(500 MHz, pyridine-d5) and 13C NMR (125 MHz, pyridine-d5) spec-
troscopic data, see Tables 1–4. ESIMS (positive) m/z 1333 [M+Na]+.
HRESIMS (positive) m/z 1333.6007 [M+Na]+ (calcd. for
C61H98O30Na, 1333.6041).
References
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triterpenoid saponins from Ardisia japonica. J. Nat. Prod. 70, 179–187.
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4.9. Impatienoside F (13)
Amorphous solid, ½a D22
ꢀ
+11.5 (c 0.80, MeOH); IR (KBr) kmax:
3398, 2925, 1720, 1619, 1412, 1384, 1292, 1257 cmꢁ1. For 1H
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4.10. Impatienoside G (14)
Amorphous solid, ½a D22
ꢀ
ꢁ31.0 (c 1.00, MeOH); IR (KBr) kmax
:
3408, 2944, 1735, 1629, 1449, 1388, 1364, 1222 cmꢁ1. For 1H
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[M+Na]+. HRESIMS (positive) m/z 1081.5166 [M+Na]+ (calcd. for
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1 M HCl (dioxane–H2O, 1:1, 200 lL), and 14 (5 mg) in 1 M HCl
(dioxane–H2O, 1:1, 1 mL) were separately heated at 100 °C for
2 h. After dioxane was removed, each solution was extracted with
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for soyasapogenol A, and 0.26 for echinocystic acid. The EtOAc ex-
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