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15
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In this study, ␣-chymotrypsin was successfully immobilized
through covalent-bonding onto the Fe3O4-CS nanoparticles via
EDC and NHS activation. The immobilization efficiency can be
further enhanced by central composite design and response sur-
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The predicted and experimental enzyme activities were 354
and 347 46.5 U/g-support, respectively. Besides, the Fe3O4-
CS-chymotrypsin nanoparticles exhibited a high acid-resisting
capacity in different pH for catalyze N-Ac-Phe-Gly-NH2 synthesis
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immobilized ␣-chymotrypsin was achieved. On the other hands,
this immobilized ␣-chymotrypsin retained 60% of the original
catalytic capability activity after 12 repeated recovery and uses.
It was also noticed that the ␣-chymotrypsin-Fe3O4-CS nanopar-
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catalysis N-Ac-Phe-Gly-NH2 synthesized. Taking the above results
into consideration, Fe3O4-CS nanoparticles have been proving to
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