Diterpenylquinone hybrids: Synthesis and assessment of gastroprotective mechanisms of action in human cells

Article abstract of DOI:10.3390/molecules180911044

A modern approach in the search for new bioactive molecules is the synthesis of novel chemical entities combining molecules of different biosynthetic origin presenting biological effects as single compounds. Gastroprotective compounds from South American medicinal plants, namely quinones and diterpenes, were used as building blocks to obtain hybrid diterpenylquinones. Starting from the labdane diterpene junicedric acid and two isomers, as well as from three quinones, including lapachol, 18 hybrid molecules were synthesized. Six of them are described for the first time. The potential gastroprotective mechanisms of action of the compounds were assessed in dose-response experiments using human gastric epithelial cells (AGS) and human lung fibroblasts (MRC-5). The following studies were carried out: stimulation of cell proliferation, cytoprotection against sodium taurocholate (NaT)-induced damage, synthesis of PGE2 and total reduced sulfhydryl (GSH) content. The antioxidant capacity of the compounds was determined on the inhibition of the lipoperoxidation in human erythrocyte membranes. Hybrid compounds presented activities different from those shown by the starting compounds, supporting the potential of this approach in the search for new bioactive molecules. The effects might be modulated by selective modification in the terpene or quinone moieties of the new molecules. Structure-activity relationships are discussed.

Full text of DOI:10.3390/molecules180911044

Molecules 2013, 18, 11044-11066; doi:10.3390/molecules180911044
OPEN ACCESS
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Article
Diterpenylquinone Hybrids: Synthesis and Assessment of
Gastroprotective Mechanisms of Action in Human Cells
Cristina Theoduloz 1,*, Ivanna Bravo 2, Mariano Walter Pertino 2 and
Guillermo Schmeda-Hirschmann 2
1
Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca,
Casilla 747, 3460000 Talca, Chile
2
Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales,
Universidad de Talca, Casilla 747, 3460000 Talca, Chile; E-Mails: ibravo@utalca.cl (I.B.);
mwalter@utalca.cl (M.W.P.); schmeda@utalca.cl (G.S.-H.)
* Author to whom correspondence should be addressed; E-Mail: ctheodul@utalca.cl;
Tel.: +56-71-2200-262; Fax: +56-71-2201-573.
Received: 6 August 2013; in revised form: 23 August 2013 / Accepted: 3 September 2013 /
Published: 10 September 2013
Abstract: A modern approach in the search for new bioactive molecules is the synthesis of
novel chemical entities combining molecules of different biosynthetic origin presenting
biological effects as single compounds. Gastroprotective compounds from South American
medicinal plants, namely quinones and diterpenes, were used as building blocks to obtain
hybrid diterpenylquinones. Starting from the labdane diterpene junicedric acid and two
isomers, as well as from three quinones, including lapachol, 18 hybrid molecules were
synthesized. Six of them are described for the first time. The potential gastroprotective
mechanisms of action of the compounds were assessed in dose-response experiments using
human gastric epithelial cells (AGS) and human lung fibroblasts (MRC-5). The following
studies were carried out: stimulation of cell proliferation, cytoprotection against sodium
taurocholate (NaT)-induced damage, synthesis of PGE2 and total reduced sulfhydryl (GSH)
content. The antioxidant capacity of the compounds was determined on the inhibition of
the lipoperoxidation in human erythrocyte membranes. Hybrid compounds presented
activities different from those shown by the starting compounds, supporting the potential of
this approach in the search for new bioactive molecules. The effects might be modulated
by selective modification in the terpene or quinone moieties of the new molecules.
Structure-activity relationships are discussed.

Molecules 2013, 18
11045
Keywords: diterpenylquinones; hybrid compounds; gastroprotection; human cells; labdane
diterpenes; lapachol
1. Introduction
South American traditional medicine uses plants in therapeutic practice. Several herbal crude drugs
have been shown to contain bioactive constituents that can be clearly related to their beneficial effects.
The resin of the “araucaria” tree (Araucaria araucana (Mol.) K. Koch, Araucariaceae) was used by the
Mapuche amerindians in Chile to treat gastric ulcers and to relieve stomach pain [1]. The stem bark of
the crude drug “lapacho” or “taheebo” (Tabebuia spp., Bignoniaceae) was formerly used by the
Guarani as an anti-inflammatory agent and then incorporated into Paraguayan folk medicine, to treat
cancer and wounds [2]. The naphthoquinone lapachol from Tabebuia species [3] and labdane
diterpenes from A. araucana, display gastroprotective activity in vivo [4] supporting the ethnobotanical
information. The bioactive compounds from those crude drugs (quinones and diterpenes) exert their
effect as single chemical entities. A new approach in drug design is to combine two different
molecules with individual intrinsic effects into a single new hybrid compound [5]. Little has been done
on the synthesis of products combining naturally occurring moieties arising from different biosynthetic
pathways into such hybrid molecules. Recent studies presented the synthesis, gastroprotective effect
and cytotoxicity of diterpenylnaphthoquinones [6] and the potential gastroprotective effect of novel
sesquiterpene quinone derivatives in human cells [7]. Terpenylquinones have been described from
some marine organisms and display several biological activities, including cytotoxicity and
anti-inflammatory effect [8]. New hybrid compounds containing terpene and quinone moieties have
been prepared and some of them present relevant biological effects including cytotoxicity [9,10] and
antifungal effect [11].
The study of gastroprotective activity of compounds has been traditionally carried out using
laboratory animals. However, due to social and economic pressures, the international trend has been a
gradual reduction in the use of experimental animals. The use of primary cell culture as well as
immortalized cell lines allows in vitro testing and studies of possible mechanisms of action. There are
several advantages in using cell cultures as biological models, namely: the amount of compound is
reduced to a minimum, variables are much better controlled than when working with entire animals
and the costs are substantially reduced. More advanced techniques consider the use of gastric epithelial
cells [7,12,13].
The aim of this work was to prepare new hybrid compounds using as starting molecules
gastroprotective diterpenes with the labdane skeleton and quinones. Human cell cultures were used to
assess their potential mechanisms of gastroprotective activity and compare it to that of the starting
substances. To draw some structure-activity trends, the hybrids included variations both in the quinone
as well as in the diterpene moieties.

Molecules 2013, 18
11046
2. Results and Discussion
2.1. Hybrid Compounds
A series of hybrid compounds was synthesized using as building blocks diterpenes and quinones.
The quinones included lapachol (1) and its hydrogenation products 2 and 3. The quinone part of the
molecule included an isoprenyl side chain, hydrogenated side chains with aromatic rings in the
naphthoquinone moiety or hydrogenated side chains and aromatic rings. The diterpene junicedric acid (4)
with an exocyclic double bond, its isomer 5 with an Δ8(9) double bond and the hydrogenation product
6 were used as the terpene part of the hybrid molecules. Eighteen hybrids, including six new
compounds, were synthesized (Scheme 1), differing in some structural features while maintaining
common characteristics to evaluate structure-activity trends. All compounds were characterized by
spectroscopic and spectrometric means and are in agreement with the proposed structures. The
structures of compounds 1–24 are shown in Figures 1, 2 and 3.
Scheme 1. General synthetic procedures for preparation of the hybrid compounds.
Quinones: compounds 1, 2 and 3. Diterpenes: compounds 4, 5 and 6.
O
O
OH
O
HO
O
O
+
O
O
O
O
O
HO
HO
4
7
1
O
OH
O
HO
O
O
+
O
O
O
O
O
O
HO
HO
4
9
2
O
OH
O
HO
O
O
+
O
O
O
HO
HO
4
11
3
Reagents and conditions: (a): DCC, DMAP, CH2Cl2, rt; or (b): oxalyl chloride, CH2Cl2, quinone, TEA.

Molecules 2013, 18
11047
Figure 1. Structure of the starting quinones 1–3 and diterpenes 4–6.
O
O
O
HO
HO
HO
O
O
O
1
4
2
3
OH
OH
OH
O
O
O
O
O
O
HO
HO
HO
5
6
Figure 2. Diterpenylquinone derivatives of junicedric acid 7–12 and 17β-dihydrojunicedric
acid 13–18.
O
O
12
14
16
8
2
3
8a
4a
13
17
15
O
O
11
1
4
7
6
20
O
O
9
1
4
1'
10
8
7
2
3
5
5
4'
2'
O
O
6
3'
18
5'
O
O
19
RO
RO
7: R = H; 8: R = CH3
9: R = H; 10: R = CH3
O
O
O
O
O
O
O
O
O
O
RO
RO
11: R = H; 12: R = CH3
13: R = H; 14: R = CH3
O
O
O
O
O
O
O
O
O
O
RO
RO
17: R = H; 18: R = CH3
15: R=H; 16: R = CH3

Molecules 2013, 18
Figure 3. Diterpenylquinone derivatives of Δ8(9) junicedric acid 19–24.
11048
O
O
O
O
O
O
O
O
O
O
RO
RO
19: R = H; 20: R = CH3
21: R = H; 22: R = CH3
O
O
O
O
O
RO
23: R = H; 24: R = CH3
2.2. Cytotoxicity
The cytotoxicity of all compounds was evaluated on confluent cultures of human lung fibroblasts
(MRC-5) and human gastric epithelial cells (AGS) to determine the working concentrations in the
subsequent mechanism of action experiments. Cell viability was determined by means of the neutral
red uptake assay and results are expressed as IC50 values (µM) in Table 1. Hydrogenation increases
cytotoxicity for the lapachol derivatives 2 and 3. From the starting diterpenes, Δ8(9) junicedric acid (5)
presented lower toxicity towards MRC-5 and AGS cells, with IC50 values of 834 and 666 µM,
respectively. The diterpene with higher cytotoxicity was compound 6 (IC50 values of 163 and 283 µM
for MRC-5 and AGS cells, respectively).
Among the six hybrid compounds derived from diterpene 4 (compounds 7–12), the carboxylic acid
derivative 7 resulted more cytotoxic than the parent quinone and diterpene 1 and 4, respectively. A
similar trend was observed for hybrids 9 and 11, prepared from quinones 2 and 3, and diterpene 4,
respectively. Methylation of 7, 9 and 11 led to the less cytotoxic derivatives of the series 8, 10 and 12,
indicating that the free C-19 carboxylic acid function is relevant for the cytotoxicity of this series. The
highest cytotoxicity value was found for compound 11, in which both, the prenylated side chain and
the benzene ring of the lapachol were reduced by hydrogenation.
The diterpene 5 differs from 4 in the position of the double bond, leading to different configurations
for both compounds and their hybrid derivatives. Hybrid compounds containing diterpene 5
(compounds 19–24), showed the same trend than derivatives from diterpene 4 when presenting a free
C-19 COOH function. Therefore, compounds 19, 21 and 23 were more toxic than their corresponding
C-19 COOMe derivatives 20, 22 and 24. As for the series from diterpene 4, the most cytotoxic
compound for the derivative series of 5 resulted compound 23, with the prenylated side chain and the
benzene ring of the lapachol completely reduced.
Among the hybrid compounds obtained from diterpene 6 (compounds 13–18), it was observed that
hybrids 13 and 17 bearing a carboxylic acid function at C-19, were more toxic than the corresponding
methylester derivatives 14 and 18. However, when only hydrogenation of the prenylated side chain of

Molecules 2013, 18
11049
the lapachol was present in the hybrid compounds (15 and 16), the ester derivative 16 showed higher
cytotoxicity that the corresponding carboxylic acid derivative 15.
Table 1. Cytotoxicity expressed as IC50 values (µM) towards confluent cultures of human
lung fibroblasts (MRC-5) and gastric epithelial cells (AGS) treated with the compounds 1–24
and lansoprazole.
IC50 ± SD (µM) a
Compound
MRC-5
>1000
AGS
Lapachol (1)
Dihydroprenyl lapachol (2)
571 ± 28
292 ± 12
87 ± 4
611 ± 42
307 ± 22
439 ± 26
834 ± 51
163 ± 5
221 ± 13
>1000
Dihydroprenyl-5,6,7,8-tetrahydrolapachol (3)
Junicedric acid (4)
383 ± 23
666 ± 35
283 ± 17
143 ± 6
>1000
Δ8(9) Junicedric acid (5)
17β-Dihydrojunicedric acid (6)
Lapachoyl junicedrate (7)
Lapachoyl junicedrate methyl ester (8)
Dihydroprenyl lapachol junicedrate (9)
449 ± 27
>1000
49 ± 10
659 ± 42
63 ± 4
Dihydroprenyl lapachoyl junicedrate methyl ester (10)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate(11)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate methyl ester (12)
Lapachoyl 17β-dihydrojunicedrate (13)
42 ± 2
667 ± 40
355 ± 21
727 ± 48
>1000
>1000
351 ± 29
916 ± 54
>1000
Lapachoyl 17β-dihydrojunicedrate methyl ester (14)
Dihydroprenyl lapachol 17β-dihydrojunicedrate (15)
Dihydroprenyl lapachol 17β-dihydrojunicedrate methyl ester (16)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-dihydrojunicedrate (17)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-dihydrojunicedrate
methyl ester (18)
596 ± 44
44 ± 4
643 ± 39
60 ± 5
369 ± 29
884 ± 62
Lapachoyl Δ8(9) junicedrate (19)
129 ± 9
483 ± 31
601 ± 26
848 ± 51
20 ± 2
148 ± 9
321 ± 24
278 ± 14
340 ± 20
29 ± 2
Lapachoyl Δ8(9) junicedrate methyl ester (20)
Dihydroprenyl lapachoyl Δ8(9) junicedrate (21)
Dihydroprenyl lapachoyl Δ8(9) junicedrate methyl ester (22)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl Δ8(9) junicedrate (23)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate methyl ester (24)
Lansoprazole b
>1000
>1000
316 ± 11
168 ± 8
Cells were treated during 24 h with the compounds. Cell viability was determined by means of the neutral red
a
uptake assay. Results are expressed as mean values ± SD. Each concentration was tested in quadruplicate
together with the control and repeated three times in different experiments; b Reference compound.
2.3. MRC-5 Fibroblast Proliferation
A key factor in the renewal and repair of gastric mucosa is the proliferative capacity of fibroblasts [14].
The ability of the compounds to accelerate cell proliferation and hence gastric wound healing was
determined using MRC-5 fibroblasts. The compounds were evaluated at six concentrations lower than
the IC50 values, being IC50/2 the highest concentration tested. The positive results are presented in
Figure 4. Percentual proliferative increase compared to untreated cells is shown in parentheses.
Stimulation of fibroblast proliferation was observed for the diterpene 5 (3.6%), the hybrid 11 (3.6%),

Molecules 2013, 18
11050
obtained by the combination of 4 and 3 and the derivatives from diterpene 6: 13 (7.3%), 17 (5.5 to
14.5%) and 18 (3.6%). The active compounds 11, 17 and 18 have in common the quinone 3 in their
structures. These compounds differ from the inactive derivatives 23 and 24 (also containing the
quinone 3) by the more planar configuration of the diterpene moiety of compounds 23 and 24. None of
the hybrid derivatives that showed activity contain the diterpene moiety 5.
Figure 4. Stimulating effect of the diterpene 5 and derivatives 11, 13, 17 and 18 on the
proliferation of MRC-5 cells. Each value represents the mean ± SD of three different
experiments in quadruplicate. ANOVA followed by Dunnett´s test. * p < 0.05 compared to
the control group.
*
*
120
100
80
60
40
20
0
*
*
*
*
*
*
*
*
2.4. Sodium Taurocholate (NaT)-Induced Damage
The model of AGS cells damaged by NaT (bile salt) was used to assess the gastroprotective effect
of compounds against the gastric ulcer induced by bile reflux [15]. Results are presented as a reduction
in cell viability. Treatment of the cells with 10 mM NaT during 30 min caused a 48% reduction of cell
viability compared with untreated controls. Cells were pre-treated during 60 min with the compounds
at 1/2, 1/4 and 1/8 of the respective IC50 values and then 10 mM NaT was added to all wells. The
reference compound sucralfate at 4 mg/mL (580 µM) showed a reduction of 35% in cell viability. The
positive results are shown in Figures 5, 6 and 7.

Molecules 2013, 18
11051
Figure 5. Effect of pre-treatment during 60 min with the reference compound sucralfate
(Suc), the quinones 1 and 2 and the diterpenes 5 and 6 followed by an incubation during
30 min with 10 mM NaT on the viability of AGS cells determined by the neutral red
uptake assay. Each value represents the mean ± SD of three different experiments in
quadruplicate. ANOVA followed by Dunnett´s test. * p < 0.05 compared to NaT group.
100
*
*
*
*
*
*
*
80
60
40
20
0
*
*
*
*
*
Figure 6. Effect of pre-treatment during 60 min with the reference compound sucralfate
(Suc) and derivatives 13, 15, 16 and 17 from 17β-dihydrojunicedric acid, followed by an
incubation during 30 min with 10 mM NaT on the viability of AGS cells determined by the
neutral red uptake assay. Each value represents the mean ± SD of three different
experiments in quadruplicate. ANOVA followed by Dunnett’s test. * p < 0.05 compared to
NaT group.
100
*
*
80
60
40
20
0
*
*
*
*
*
*
*
*

Molecules 2013, 18
11052
Figure 7. Effect of pre-treatment during 60 min with the reference compound sucralfate
(Suc) and Δ8(9) junicedric acid derivatives 19, 21 and 23 followed by an incubation during
30 min with 10 mM NaT on the viability of AGS cells determined by the neutral red
uptake assay. Each value represents the mean ± SD of three different experiments in
quadruplicate. ANOVA followed by Dunnett’s test. * p < 0.05 compared to NaT group.
100
*
*
80
*
*
*
*
*
*
*
60
40
20
0
The results showed significant cytoprotective effect (p < 0.05) for the starting quinones 1 (25, 22
and 29% cell protection at 285, 143 and 71 µM, respectively) and 2 (27, 23 and 23%, at 146, 73 and 37
µM respectively), the diterpenes 5 (35, 25 and 19% reduction in cell viability at 333, 167 and 83 µM,
respectively) and 6 (25% cell viability reduction at 71 and 35 µM) (Figure 5).
Cytoprotection was observed for the diterpene 6 hybrids 13, 15–17 (Figure 6). The effect of
compound 17 at 30, 15 and 7.5 µM was in the same range as sucralfate (34%–36%), while the activity
of 13 at 89 and 45 µM was 38% and the effect of 16 at 81 µM was 44%, compared to NaT controls.
Best effect, even higher than that of sucralfate, was found for the hybrid 15 at 500, 250 and 125 µM,
reducing cell viability by 21, 27 and 27%, respectively.
The hybrids of the diterpene 5 with 1, 2 and 3 (compounds 19, 21 and 23) were active (Figure 7).
However, the corresponding methyl esters were devoid of effect, suggesting that the free COOH at
C-19 is needed for cytoprotection. Even if COOH is needed for cytoprotection, the carboxylic acid
derivatives were the most toxic compounds. The cytoprotection effect might be due to the implication
of OH residues in radical mechanisms with ROS species, and that might be the reason why the methyl
ester derivatives, lacking of OH, do not present cytoprotective effects. Compound 19 reduced cell
viability by 40, 27 and 15% at 74, 37 and 19 µM. Compound 21 at 139, 70 and 35 µM improved
viability by 30, 27 and 25%, while the effect of compound 23 at 15 and 7.5 µM was 39 and 32%,
respectively. The inverse dose-response effect of compounds 5 and 19 and in a less extent for 21 might
be related to the solubility of the compounds.

Molecules 2013, 18
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Cytoprotective compounds may protect against NaT-induced damage forming a physical barrier or
binding bile salts, interacting with cell membranes or modifying the expression of trefoil factor family
2 mRNA and c-fos protein [16,17].
2.5. Effect on Prostaglandin E2 (PGE2) Content
A relevant gastroprotective mechanism involves the prostaglandins that accelerate ulcer healing,
epithelial cell proliferation, synthesis of growth factors and inhibition of inflammatory cell infiltration [18].
The prostaglandin E2 (PGE2) is considered to be the major humoral factor providing resistance to the
gastric mucosa [19]. Some terpenes and their derivatives exert their gastroprotective effect
increasing the gastric PGE2 synthesis in vitro and in vivo [7,20]. Experiments were carried out using
post-confluent AGS cells. The results are presented in Table 2. Among the starting quinones and
diterpenes 1–6, only lapachol 1 stimulated PGE2 synthesis in AGS, cells showing a 2.5- and 2.3-fold
increase in PGE2 content at 286 and 143 µM, respectively. Compound 8 at 500 and 250 µM elicited a
15.2- and 5.2-fold increase of PGE2 content, respectively.
Table 2. Effect of lapachol 1 and the diterpenyl naphthoquinones 8–10, 15, 18, 21, 22 and
24 on the total PGE2 content of post-confluent AGS cells treated during 1 h with the
compounds at 1/2 and 1/4 of IC50.
Concentration
PGE2
Compound
(µM)
-
(pg/mL)
Control
52 ± 5
Indomethacin a
Lapachol (1)
100
286
143
500
250
12.5
323
162
500
250
Not detected
132 ± 12 *
117 ± 10 *
787 ± 56 *
272 ± 30 *
309 ± 29 *
465 ± 39 *
306 ± 32 *
372 ± 25 *
279 ± 20 *
Lapachoyl junicedrate methyl ester (8)
Dihydroprenyl lapachol junicedrate (9)
Dihydroprenyl lapachoyl junicedrate methyl ester (10)
Dihydroprenyl lapachol 17β-dihydrojunicedrate (15)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-dihydrojunicedrate
methyl ester (18)
221
156 ± 13 *
111
85
123 ± 9 *
162 ± 18 *
114 ± 11 *
123 ± 12 *
Dihydroprenyl lapachoyl Δ8(9) junicedrate (21)
Dihydroprenyl lapachoyl Δ8(9) junicedrate methyl ester (22)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate methyl ester (24)
170
250
Each value represents the mean ± SD of two different experiments in triplicate. ANOVA followed by
Dunnett’s multiple comparison test. * p < 0.01 compared to control group. a Reference compound.
The dihydroprenyl lapachol hybrids 9–10, 15, 21–22, bearing the same quinone moiety but
differing in the identity of the terpene, increased PGE2 synthesis as follows: 9 (12.5 µM, 5.9-fold), 10
(323 and 162 µM, 8.9- and 5.9-fold), 15 (500 and 250 µM, 7.2 and 5.4-fold), 21 (85 µM, 3.1-fold) and 22
(170 µM, 2.2-fold), respectively.

Molecules 2013, 18
11054
PGE2 synthesis was also stimulated by compounds 18 (221 and 111 µM, 3.0 and 2.4-fold,
respectively) and 24 (250 µM, 2.4-fold). The hybrids 18 and 24 have in common the quinone moiety 3
and differ in the identity of the diterpene part of the hybrid molecule.
2.6. Effect on Cellular Reduced Glutathione (GSH) Content
Generation of gastric ulcers has been related to the effect of free radicals. This fact explains the
ulcerogenic effect of ethanol on the gastric mucosa. Intracellular GSH content is a determining factor
that contributes to the protection of the gastric mucosa against oxidative stress [21]. Experiments were
carried out using post-confluent AGS cells. The known stimulant of GSH synthesis, N-acetyl-L-
cysteine (NAC) was used as positive control. Under our experimental conditions, NAC (750 µM)
increased GSH content by 30% compared to untreated controls. Results are summarized in Table 3.
Among the starting diterpenes, junicedric acid (4) and 17β-dihydrojunicedric acid (6) significantly
(p < 0.05 and p < 0.01) stimulated GSH production. Best activity was observed for compound 6
(60 and 43% increase at 71 and 37 µM, respectively). Among the hybrid compounds, a good effect
was found for the junicedric acid-lapachol derivatives 7 (23 and 18% at 36 and 18 µM, respectively)
and 8 (24 and 19% at 250 and 125 µM, respectively) as well as for dihydroprenyl lapachol junicedrate
9 (16 and 15% at 12 and 6 µM, respectively).
Considering the diterpene 6 hybrids, best activity was found for the derivative 15 (35 and 31% at
250 and 125 µM, respectively), 17 (51 and 89% at 15 and 7.5 µM, respectively) and 18 (26% both at
221 and 111 µM). Compounds 17 and 18 differ in the free COOH (17) or COOCH3 function (18) in
the diterpene moiety.
Most of the Δ8(9) junicedric acid (diterpene 5) hybrids presented activity. However, the starting
diterpene was inactive. In this group of compounds, effect was observed for the derivatives with
lapachol (compound 20, 13 and 19% at 80 and 40 µM, respectively), dihydroprenyl lapachol
(compound 21, 33% at 70 µM) and dihydroprenyl-5,6,7-8-tetrahydrolapachol (compound 23, 26% at
7.3 µM). Inverse dose-response effect was observed for the hybrids 17, 20 and 24. This effect might be
related to the solubility of the compounds.
Table 3. Total reduced sulfhydril (GSH) content in post-confluent AGS cells treated
during 4 h with N-acetyl-L-cysteine (NAC) and the compounds 1–24 at 1/4 and 1/8 of IC50.
Concentration
GSH
(nmol/106 cells)
13.6 ± 0.7
Compound
(µM)
-
Control
NAC a
750
143
72
* 17.7 ± 1.4
14.1 ± 0.8
Lapachol(1)
13.9 ± 0.9
Dihydroprenyl lapachol(2)
73
13.8 ± 0.7
37
13.6 ± 0.7

Molecules 2013, 18
11055
Table 3. Cont.
Concentration
GSH
Compound
(µM)
22
(nmol/106 cells)
13.0 ± 0.5
Dihydroprenyl-5,6,7,8-tetrahydrolapachol (3)
Junicedric acid (4)
11
14.3 ± 0.7
96
* 17.2 ± 1.0
* 18.4 ± 1.1
14.1 ± 0.6
48
Δ8(9) Junicedric acid (5)
166
83
13.6 ± 0.5
17β-Dihydrojunicedric acid (6)
71
** 21.7 ± 1.3
* 19.5 ± 0.9
* 16.7 ± 0.7
16.1 ± 0.9
37
Lapachoyl junicedrate (7)
36
18
Lapachoyl junicedrate methyl ester (8)
Dihydroprenyl lapachol junicedrate (9)
Dihydroprenyl lapachoyl junicedrate methyl ester (10)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate (11)
250
125
12
* 16.8 ± 0.9
* 16.2 ± 0.6
* 15.8 ± 0.6
* 15.6 ± 0.6
13.0 ± 0.7
6
165
83
13.4 ± 0.7
16
13.2 ± 0.5
8
13.1 ± 0.6
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate
250
13.9 ± 0.8
methyl ester (12)
125
88
13.7 ± 1.0
11.4 ± 0.6
11.9 ± 0.4
12.7 ± 0.4
14.4 ± 0.5
* 18.4 ± 1.2
* 17.8 ± 0.9
14.1 ± 0.6
14.9 ± 0.7
Lapachoyl 17β-dihydrojunicedrate (13)
Lapachoyl 17β-dihydrojunicedrate methyl ester (14)
Dihydroprenyl lapachol 17β-dihydrojunicedrate (15)
Dihydroprenyl lapachol 17β-dihydrojunicedrate methyl ester (16)
44
229
115
250
125
160
80
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-
dihydrojunicedrate (17)
15
7.5
221
** 20.5 ± 1.0
** 25.8 ± 1.6
* 17.2 ± 1.0
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-
dihydrojunicedrate methyl ester (18)
111
37
19
80
40
70
35
* 17.1 ± 1.1
13.5 ± 0.5
Lapachoyl Δ8(9) junicedrate (19)
13.1 ± 0.4
Lapachoyl Δ8(9) junicedrate methyl ester (20)
Dihydroprenyl lapachoyl Δ8(9) junicedrate (21)
* 15.4 ± 1.3
* 16.2 ± 1.0
* 18.1 ± 1.1
13.3 ± 0.7

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Table 3. Cont.
Concentration
GSH
Compound
(µM)
85
(nmol/106 cells)
14.2 ± 0.6
Dihydroprenyl lapachoyl Δ8(9) junicedrate methyl ester (22)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl Δ8(9) junicedrate (23)
43
13.6 ± 0.9
7.3
3.6
* 17.1 ± 1.3
12.6 ± 0.8
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate
250
* 18.0 ± 0.9
methyl ester(24)
125
* 18.7 ± 1.0
a
NAC: reference compound. Each value represents the mean ± SD of three different experiments in
triplicate. ANOVA followed by Dunnet’s test. * p < 0.05; ** p < 0.01.
2.7. Inhibition of Lipoperoxidation
Antioxidant effect of the compounds was assessed using human erythrocyte membranes. The
products were tested at 100 µg/mL and results are presented in Table 4. Among the starting quinones,
the hydrogenation products of lapachol 2 and 3 were more active than lapachol (1), with IC50 values of
25.4 and 33.3 µg/mL, respectively. From the diterpene moieties, only Δ8(9)-junicedric acid (5) was
active with an IC50 of 43.8 µg/mL. The inhibition of lipoperoxidation for the hybrids prepared from the
diterpene 4 (compounds 7–12) was moderate. Better effects were found for the diterpene 4 derivative
with lapachol and a COOCH3 at C-19 (8, IC50: 81.4 µg/mL), and the hybrids containing 4 and the
quinone 2, either with a free C-19 carboxylic acid (9, IC50: 40.1 µg/mL) or as the methyl ester (10,
IC50: 99.4 µg/mL). Among the hybrids of diterpene 6 (compounds 13–18), best antioxidant activity
was found for: 13, 14 and 15 with IC50 values of 61.1, 19.7 and 25.3 µg/mL, respectively. The three
compounds contain the quinone 1 or 2 in the hybrid structure. The compounds 19–24 were prepared
using Δ8(9) junicedric acid (5) as the diterpene moiety and presented moderate antioxidant effect, best
activity was displayed by 20 (IC50: 58.1 µg/mL) and 23 (IC50: 59.5 µg/mL). In the same assay, the
reference compound catechin presented an IC50 value of 75.4 µg/mL.
Structural diversity is a requirement to draw structure-activity relationships/trends in compounds
series. The mechanisms of action of the starting diterpenes and quinones were compared with those of
the new hybrids. The starting compounds showed activity in one or two (exceptionally three) out of the
mechanisms of action investigated. Diterpene 5 stimulates cell proliferation, while none of its hybrids
presented effect. From the four hybrids of diterpenes 4 and 6 with the quinone 3, three were active.
The quinones 1–2 and diterpenes 5–6 were active in the NaT assay. Hybrids from diterpenes 5 and
6 with quinones show effects on NaT when the COOH group at C-19 of the diterpene is free
(compounds 13, 15, 17, 19, 21 and 23). The corresponding methyl esters (except compound 16) were
devoid of effect. The products prepared from the diterpene 4, with a 8(17) double bond were inactive.
Among the parent molecules, only lapachol increased PGE2 synthesis. Relevant changes in PGE2
levels were observed for hybrid compounds, with several products stimulating synthesis. Regardless of
the diterpene moiety, hybrids with dihydroprenyl lapachol 2 and two methyl esters from the hybrids
with 3 were active.

Molecules 2013, 18
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In the GSH assay, quinones were inactive, but the diterpenes 4 and 6 presented effect. For the
hybrids of diterpene 4, the activity is observed for the products with a planar naphthoquinone (7–9) but
it is lost when the aromatic ring of the quinone is hydrogenated. The diterpene 5, with a double bond
Δ8(9) has a more planar configuration and was devoid of activity. However, four out of the six hybrids
from 5 proved to be active. Most of them presented the free COOH function at C-19.
Table 4. Effect of compounds 1–24 on the inhibition of the lipoperoxidation in human
erythrocyte membranes. a Percent effect at 100 µg/mL or IC50 values (µg/mL).
% Inhibition
of the lipoperoxidation a
Compound
Lapachol (1)
Dihydroprenyl lapachol (2)
38
IC50 25.4 ± 2.4 µg/mL
Dihydroprenyl-5,6,7,8-tetrahydrolapachol (3)
Junicedric acid (4)
IC50 33.3 ± 2.3 µg/mL
25
Δ8(9) Junicedric acid (5)
IC50 43.8 ± 2.8 µg/mL
17β-dihydrojunicedric acid (6)
30
Lapachoyl junicedrate (7)
10
Lapachoyl junicedrate methyl ester (8)
IC50 81.4 ± 11.0 µg/mL
Dihydroprenyl lapachol junicedrate (9)
IC50 40.1 ± 5.3 µg/mL
Dihydroprenyl lapachoyl junicedrate methyl ester (10)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate (11)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate methyl ester (12)
Lapachoyl 17β-dihydrojunicedrate (13)
IC50 99.4 ± 9.1 µg/mL
44
37
IC50 61.1 ± 5.9 µg/mL
IC50 19.7 ± 1.4 µg/mL
IC50 25.3 ± 4.1 µg/mL
46
Lapachoyl 17β-dihydrojunicedrate methyl ester (14)
Dihydroprenyl lapachol 17β-dihydrojunicedrate (15)
Dihydroprenyl lapachol 17β-dihydrojunicedrate methyl ester (16)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-dihydrojunicedrate (17)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-dihydrojunicedrate methyl
ester (18)
48
28
Lapachoyl Δ8(9) junicedrate (19)
49
Lapachoyl Δ8(9) junicedrate methyl ester (20)
Dihydroprenyl lapachoyl Δ8(9) junicedrate (21)
Dihydroprenyl lapachoyl Δ8(9) junicedrate methyl ester (22)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl Δ8(9) junicedrate (23)
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl junicedrate methyl ester (24)
Catechin b
IC50 58.1 ± 4.1 µg/mL
Nd
46
IC50 59.5 ± 4.3 µg/mL
27
IC50 75.4 ± 6.0 µg/mL
b
Results are expressed as mean values ± SD of three different experiments in triplicate. Reference
compound. Nd: not determined due to turbidity.
Among the starting compounds, the quinones 2 and 3 as well as the diterpene 5, displayed inhibitory
activity in the lipoperoxidation assay. Best antioxidant effect was found for hybrids from the diterpene 6
either with the quinone 1 or 2 or with weaker effect for diterpene 6 hybrids with the same quinones.
In a previous report, part of the compounds presented in this study, were evaluated for
gastroprotective effect by the HCl-EtOH-induced gastric lesions model in mice [6].The compounds
were administered orally at a single dose of 5 mg/kg. Compounds 9–10 and 21–22 displayed

Molecules 2013, 18
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significant activity in vivo [6]. In the present study, these compounds stimulated the PGE2 synthesis in
AGS cells. Our results show that compounds 8 and 20, active in vivo, increased GSH content in AGS
cells and protected erythrocyte membranes against induced lipoperoxidation. These in vitro findings
sustain the in vivo observations suggesting that different and complementary mechanisms may be
underlying the gastroprotective effect of the compounds.
Gastric lesions are a consequence of an imbalance between defensive and aggressive factors in the
gastric mucosa. Increase in the defensive factors, namely: restitution of gastric epithelium, protection
against damage induced by bile salts and free radicals, increase in PGE2 levels, GSH, bicarbonate and
others, may reduce the appearance of gastric lesions in animals [7,16,17]. It has been shown that
gastroprotective compounds exert their effects mainly stimulating the defensive factors in the gastric
mucosa rather than inhibiting the aggressive agents (gastric acid or pepsin) [16,17,22].
In this work the possible mechanisms of gastroprotective effect of diterpenylquinones were
assessed using human lung fibroblasts (MRC-5) and gastric epithelial cells (AGS). The evaluation of
the compound activity on some defensive factors depending on gastric epithelial cells has been carried
out using AGS cells. This cell line has been successfully used in the study of different aspects related
to the gastric mucosa [7,23,24]. The hybrid compounds investigated stimulated one or more of the
defensive factors described as relevant to protect the gastric mucosa. To obtain hybrid molecules with
better activity than the parent compounds, structural modifications should be undertaken, followed by
a suitable combination of substituents placed at the appropriate positions of hybrid compounds [25].
To understand the relationship between in vitro and in vivo activity, further research should be carried
out, since factors such as absorption, bioavailability, location of the targets in different tissues or
organs, receptor and enzyme densities will influence in effect [26].
3. Experimental
3.1. General Experimental Procedures
Optical rotations were obtained for solutions in CHCl3 (concentrations expressed in g/100 mL) on a
Jasco DIP 370 polarimeter (Jasco Analytical Instruments, Easton, MD, USA). IR spectra were
recorded on a Nicolet Nexus FT-IR instrument (Thermo Electron Corporation, Waltham, MA,
USA).1H-NMR spectra were recorded at 400 MHz and 13C-NMR data were obtained at 100 MHz on a
Bruker Avance spectrometer (Bruker, Rheinstetten, Germany). Chemical shifts are given in δ (ppm)
with TMS as the internal standard. Mass spectra were measured in a LC/MSD-TOF (Agilent
Technologies) by the electrospray technique. Positive or negative ion mode was used according to
the samples. As internal reference in the ESI (+) mode, purine (m/z 121.0509) and HP-0921
(m/z 922.0098) were used. In the negative mode: ESI (−), purine (m/z 112.9856) and HP-0921
(m/z 1033.9881) were used as internal references. Silica gel 60 (Merck, 63–200 µm particle size) was
used for column chromatography, precoated silica gel plates (Merck, Kieselgel 60 F254, 0.25 mm)
were used for thin layer chromatography (TLC). TLC spots were visualized by spraying the
chromatograms with p-anisaldehyde-ethanol-acetic acid-H2SO4(2:170:20:10 v/v) and heating at 110 °C
for 3 min. 1,3-Dicyclohexylcarbodiimide (DCC) and dimethylaminopyridine (DMAP) were from
Merck (Schuchardt, Germany). Oxalyl chloride was from Sigma-Aldrich (St. Louis, MO, USA).

Molecules 2013, 18
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3.2. General Procedure for the Synthesis of Hybrid Compounds
3.2.1. Quinones
The naphthoquinone lapachol (1) was isolated from the wood of “lapacho” tree (Tabebuia sp). The
hydrogenation products of lapachol (compounds 2 and 3) were prepared trating (1) in EtOAc with
Pd/C [6]. The synthetic scheme for the synthesis is presented in [6].
3.2.2. Diterpenes
The diterpene 4 was obtained from the resin of Araucaria araucana as previously reported [6]. The
isomers 5 and 6 were prepared from 4. Briefly, treatment of 4 with HBr in acetic acid afforded Δ8(9)
junicedric acid (5) while catalytic hydrogenation of 4 in ethyl acetate with 10% Pd/C in a 1:10 molar
ratio yielded the hydrogenation product 6. The synthetic scheme for the synthesis is shown in [6].
3.2.3. Hybrid Compounds
The compounds 7–14 and 19–22 were synthesized according to [6]. The new compounds
15 and 17 were prepared by treating the diacid 6 (1 mEq) in dry CH2Cl2 (DCM) with
1,3-dicyclohexylcarbodiimide (DCC) (1 mEq) at room temperature under constant stirring. After 10
min, the quinones 2 and 3 (1 mEq) dissolved in dry DCM, were added together with a catalytic amount
of dimethylaminopyridine (DMAP). After 2–4 h, the reaction was stopped by adding water and
extracted with DCM. Compound 23 was synthesized from the diacid 5 under an inert (N2) atmosphere.
Briefly, the diterpene 5 was dissolved in dry CH2Cl2 (DCM) and ice cooled under nitrogen flow. To
this solution, oxalyl chloride in dry DCM was added drop wise with stirring. The diterpene: oxalyl
chloride molar ratio was 1:40. The mixture was stirred at room temperature overnight, then the DCM
was evaporated and the residue vacuum dried. The dry residue was dissolved in dry DCM and the
quinone 3 was added as well as triethylamine (TEA) under constant N2 flow. The diterpene acyl
chloride-quinone-TEA molar ratio was 1:1:2. The reaction mixture was left at room temperature under
stirring and inert atmosphere for two days. Then, the mixture was washed two times with water, and
the aqueous phase extracted with DCM to obtain the crude reaction mixture.
The reaction mixtures were dried over Na2SO4. Purification was carried out using a combination of
gel permeation in Sephadex LH-20 with a MeOH:DCM 1:1 (v/v) mixture, or silica gel column
chromatography using PE:EtOAc mixtures (90:10, 85:15, 80:20) (v/v), to afford compounds 15, 17 and
23 in 14.3, 13.7 and 17% w/w yields, respectively. The methyl esters 16, 18, and 24 were obtained
treating the compounds 15, 17 and 23 with diazomethane in diethyl ether, with 92%–98% w/w yield.
1
The purity of all the derivatives was over 95%, as determined by H-NMR spectroscopy. The new
compounds 15–18, 23 and 24 are described below.
Dihydroprenyl lapachol 17β-dihydrojunicedrate (15): 17β-Dihydrojunicedric acid (6, 246.8 mg,
0.732 mmol), DCC (302 mg, 1.464 mmol), a catalytic amount of DMAP and dihydroprenyl lapachol
(2, 177.9 mg, 0.732 mmol), were stirred at room temperature in dry CH2Cl2 (20 mL) for 2–4 h. The
reaction mixture was cooled in an ice bath. After addition of water, the aqueous phase was extracted
with EtOAc (3 × 20 mL). The extract was dried over anhydrous Na2SO4 and taken to dryness under

Molecules 2013, 18
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reduced pressure. Purification was carried out using a combination of gel permeation in Sephadex
LH-20 with a MeOH-DCM 1:1 (v/v) mixture and silica gel column chromatography using PE-EtOAc
20
mixtures 90:10 (v/v), to afford compound 15 (60.7 mg, 14.3%): yellow resin; [α]D +2.35 (c 0.085
1
CHCl3); IR νmax (film) 2957, 2931, 2854, 1725, 1675, 1463, 1386, 1287, 1074, 998 cm−1; H-NMR
(CDCl3): δ 8.11 (2H, m, quinone H-5 and H-8), 7.75 (2H, m, quinone H-6 and H-7), 2.74 (1H, dd,
J = 16, 4 Hz, H-14 α), 2.55 (2H, t, J = 8 Hz, H-1'), 2.42 (1H, m, H-14 β), 1.61 (1H, m, H-3'), 1.45-1.52
(2H, m, H-2'), 1.27 (3H, s, H-18), 1.13 (3H, d, J = 6.8 Hz, H-16), 0.93 (6H, d, J = 6.4 Hz, H-4' and H-5'),
0.90 (3H, d, J = 6.4 Hz, H-17), 0.84 (3H, s, H-20); 13C-NMR (CDCl3): 39.61 (C-1), 19.43 (C-2), 37.45
(C-3), 45.46 (C-4), 52.82 (C-5), 23.00 (C-6), 34.94 (C-7), 29.00 (C-8), 57.64 (C-9), 38.84 (C-10),
18.84 (C-11), 35.12 (C-12), 30.79 (C-13), 40.72 (C-14), 170.29 (C-15), 20.10 (C-16), 15.17 (C-17),
28.04 (C-18), 178.08 (C-19), 14.89 (C-20); Quinone: 173.42, 184.49 (C-1 and C-4), 151.04 (C-2),
140.09 (C-3), 130.89, 132.08 (C-4a, C-8a), 126.61, 126.50 (C-5 and C-8), 133.95, 133.72 (C-6 and C-7),
22.40 (C-1'), 37.00 (C-2'), 28.34 (C-3'), 22.28 (C-4'), 22.28 (C-5'); EIMS m/z 576 [M]+ (2), 293 (17),
244 (20), 243 (20), 242 (100), 227 (27), 123 (28), 109 (14), 95 (10), 81 (11), 55 (11).
20
Dihydroprenyl lapachol 17β-dihydrojunicedrate methyl ester (16). Yellow resin; [α]D +20 (c 0.056,
CHCl3); IR νmax (film) 2951, 2868, 2844, 1772, 1725, 1679, 1463, 1380, 1300, 1151, 1081, 941, 712 cm−1;
1H-NMR (CDCl3): δ 8.11 (2H, m, quinone H-5 and H-8), 7.74 (2H, m, quinone H-6 and H-7), 3.65
(3H, s, OCH3), 2.75 (1H, dd, J = 14.8, 5.2 Hz, H-14 α), 2.55 (2H, t, J = 8 Hz, H-1'), 2.44 (1H, dd,
J = 15.2, 8.8 Hz, H-14 β), 1.61 (1H, m, H-3'), 1.45-1.52 (2H, m, H-2'), 1.19 (3H, s, H-18), 1.13 (3H, d,
J = 6.4 Hz, H-16), 0.95 (6H, d, J = 6.8 Hz, H-4' and H-5'), 0.92 (3H, d, J = 7.6 Hz, H-17), 0.70 (3H, s,
H-20); 13C-NMR (CDCl3): 40.23 (C-1), 19.47 (C-2), 38.55 (C-3), 44.31 (C-4), 53.31 (C-5), 23.43 (C-6),
35.35 (C-7), 29.66 (C-8), 57.89 (C-9), 39.08 (C-10), 19.28 (C-11), 35.62 (C-12), 31.27 (C-13), 41.18
(C-14), 170.79 (C-15), 20.57 (C-16), 15.34 (C-17), 29.26 (C-18), 178.51 (C-19), 14.68 (C-20), 51.55
(OCH3); Quinone: 173.26, 184.95 (C-1 and C-4), 151.5 (C-2), 140.54 (C-3), 131.32, 132.52 (C-4a,
C-8a), 127.06, 126.95 (C-5 and C-8), 134.41, 134.18 (C-6 and C-7), 22.86 (C-1'), 37.90 (C-2'), 28.79
(C-3'), 22.74 (C-4'), 22.74 (C-5'); molecular formula: C36H50O6 (578.3607). m/z 596.3947 [M+NH4]+,
error: 0.20 ppm with the empirical formula C36H54NO6; m/z 601.3499 [M+Na]+, error: 0.08 ppm with
the empirical formula C36H50O6Na.
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-dihydrojunicedrate (17). 17β-Dihydrojunicedric acid
(6, 229.2 mg, 0.679 mmol), DCC (280.2 mg, 1.358 mmol), a catalytic amount of DMAP and
dihydroprenyl-5,6,7,8-tetrahydrolapachol (3, 168.4 mg, 0.679 mmol), were stirred at room temperature
in dry CH2Cl2 (20 ml) for 2–4 h. The reaction mixture was cooled in an ice bath. After addition of
water, the aqueous phase was extracted with EtOAc (3 × 20 mL). The extract was dried over
anhydrous Na2SO4 and taken to dryness under reduced pressure. The residue was purified by silica gel
column chromatography, eluting with an hexane/EtOAc gradient (95:5, 91:9, 87:13), yielding 17
20
(52.9 mg, 13.7%): amber resin; [α]D +23 (c 0.065, CHCl3); IR νmax (film) 2959, 1769, 1699, 1662,
1
1625, 1549, 1467, 1455, 1382, 1269, 1205, 1077, 913, 755 cm−1; H-NMR (CDCl3): δ 2.68 (1H, dd,
J = 14.8, 5.2 Hz, H-14 α), 2.40 (2H, m, quinone H-5 and H-8), 2.28 (1H, m, H-14 β), 1.70 (2H, m,
quinone H-6 and H-7), 1.59 (2H, m, H-1'), 1.48 (2H, m, H-2'), 1.25 (3H, s, H-18), 1.10 (3H, d, J = 6.8
Hz, H-16), 0.92 (6H, d, J = 6.8 Hz, H-4' and H-5'), 0.92 (3H, d, J = 6.4 Hz, H-17), 0.80 (3H, s, H-20);

Molecules 2013, 18
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13C-NMR (CDCl3): 40.14 (C-1), 19.22 (C-2), 38.31 (C-3), 44.20 (C-4), 53.34 (C-5), 23.41 (C-6), 35.36
(C-7), 29.67 (C-8), 57.86 (C-9), 39.30 (C-10), 18.33 (C-11), 35.60 (C-12), 31.24 (C-13), 41.13 (C-14),
170.97 (C-15), 20.51 (C-16), 15.36 (C-17), 29.45 (C-18), 180.70 (C-19), 14.79 (C-20); Quinone:
184.36, 187.37 (C-1 and C-4), 149.02 (C-2), 141.29 (C-3), 137.32, 143.20 (C-4a, C-8a), 23.22, 21.45
(C-5 and C-8), 22.30, 21.45 (C-6 and C-7), 23.41 (C-1'), 37.90 (C-2'), 28.67 (C-3'), 22.71 (C-4'), 22.71
(C-5'); molecular formula: C35H52O6 (568.3764). m/z 586.4086 [M+NH4]+, error: 0.20 ppm with the
empirical formula C35H56NO6.
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl 17β-dihydrojunicedrate methyl ester (18). Yellow resin;
20
[α]D +43 (c 0.014 g/100 mL CHCl3); IR νmax (film) 2956, 2846, 1772, 1729, 1662, 1464, 1452, 1385,
1208, 1193, 1151, 1077, 910 cm−1; 1H-NMR (CDCl3): 21 δ 3.57 (3H, s, OCH3), 2.60 (1H, dd, J = 14.8,
5.2 Hz, H-14 α), 2.31 (2H, m, quinone H-5 and H-8), 2.30 (1H, m, H-14 β), 2.07 (1H, d, J = 12.4 Hz,
H-13), 1.62 (2H, m, quinone H-6 and H-7), 1.53 (2H, m, H-1'), 1.10 (3H, s, H-18), 1.01 (3H, d, J = 6.8 Hz,
H-16), 0.84 (6H, d, J = 6.8 Hz, H-4' and H-5'), 0.82 (3H, d, J = 7.2 Hz, H-17), 0.60 (3H, s, H-20);
13C-NMR (CDCl3): 40.22 (C-1), 19.46 (C-2), 38.55 (C-3), 44.32 (C-4), 53.31 (C-5), 23.42 (C-6), 35.34
(C-7), 29.65 (C-8), 57.89 (C-9), 39.08 (C-10), 19.27 (C-11), 35.62 (C-12), 31.26 (C-13), 41.13 (C-14),
170.97 (C-15), 20.52 (C-16), 15.33 (C-17), 29.26 (C-18), 178.53 (C-19), 14.66 (C-20), 51.54 (OCH3);
Quinone: 180.73, 187.37 (C-1 and C-4), 149.03 (C-2), 141.30 (C-3), 137.33, 143.21 (C-4a, C-8a),
23.07, 21.45 (C-5 and C-8), 22.30, 21.45 (C-6 and C-7), 23.23 (C-1'), 37.91 (C-2'), 28.68 (C-3'), 22.70
(C-4'), 22.70 (C-5'); molecular formula: C36H54O6 (582.3920). m/z 600.4242 [M+NH4]+, error: 2.85
ppm with the empirical formula C36H58NO6.
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl Δ8(9) junicedrate (23). Δ8(9) Junicedric acid (5, 228 mg,
0.678 mmol), oxalyl chloride (3.44 mg, 27.12 mmol), dihydroprenyl lapachol (2, 0.168 mg, 0.678 mmol)
and triethylamine (TEA) (0.137 mg, 1.356 mmol) were dissolved in dry CH2Cl2 (DCM) under nitrogen
flow. The reaction mixture was left at room temperature under stirring and inert atmosphere for two
days. Then, the mixture was washed two times with water, and the aqueous phase extracted with DCM
to obtain the crude reaction mixture. The extracts were dried over Na2SO4. Purification was carried out
using silica gel column chromatography using PE:EtOAc 90:10 (v/v), to afford compound 23 (65.4 mg,
20
17%): orange resin; [α]D +31 (c 0.036, CHCl3); IR νmax (film) 2954, 2864, 1772, 1695, 1662, 1619,
1
1469, 1380 1263, 1207, 1134, 1114, 1077, 911 cm−1; H-NMR (CDCl3): δ 2.61 (1H, dd,
J = 15.3, 5.5 Hz, H-14 α), 2.41 (4H, m, quinone H-5 and H-8), 2.40–2.35 (1H, m, 14 β), 2.40-2.33 (2H,
m, H-1'), 2.08 (1H, m, H-13), 1.67 (4H, m, quinone H-6 and H-7), 1.56 (3H, s, H-17), 1.53 (1H, m,
H-3'), 1.30 (2H, m, H-2'), 1.24 (3H, s, H-18), 1.08 (3H, d, J = 6.6 Hz, H-16), 0.89 (3H, d, J = 6.6 Hz,
H-4'), 0.89 (3H, d, J = 6.6, H-5'), 0.85 (3H, s, H-20), 13C-NMR (CDCl3): 37.22 (C-1), 19.89 (C-2), 37.38
(C-3), 44.18 (C-4), 53.92 (C-5), 21.45 (C-6), 37.16 (C-7), 127.18 (C-8), 139.35 (C-9), 40.13 (C-10),
26.13 (C-11), 34.62 (C-12), 31.75 (C-13), 41.35 (C-14), 170.87 (C-15), 19.88 (C-16), 20.11 (C-17),
29.04 (C-18), 180.68 (C-19), 18.29 (C-20); Quinone: 184.73, 187.36 (C-1 and C-4), 149.01 (C-2),
141.29 (C-3), 137.32, 143.21 (C-4a, C-8a), 23.22, 22.86 (C-5 and C-8), 21.45, 21.11 (C-6 and C-7),
22.28 (C-1'), 37.49 (C-2'), 28.66 (C-3'), 22.71 (C-4'), 22.71 (C-5'); molecular formula: C35H50O6
(566.3607), m/z 567.3687 [M+H]+, error: 1.11 ppm with the empirical formula C35H51O6; m/z
584.3944 [M+NH4]+, error: 0.23 ppm with the empirical formula C35H54NO6.

Molecules 2013, 18
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20
Dihydroprenyl-5,6,7,8-tetrahydrolapachoyl Δ8(9) junicedrate methyl ester (24). Yellow solid; [α]D
+22 (c 0.027, CHCl3); IR νmax (film) 2953, 2868, 1769, 1726, 1659, 1470, 1449, 1379, 1138, 1081,
916, 761 cm−1; 1H-NMR (CDCl3): δ 3.61 (3H, s, OCH3), 2.76 (1H, dd, J = 15.3, 5.5 Hz, H-14 α), 2.62
(1H, dd, J = 15.3, 8.1 Hz, H-14 β), 2.06 (1H, m, H-13), 2.40 (4H, m, quinone H-5 and H-8), 2.40-2.33
(2H, m, H-1'), 1.67 (4H, m, quinone H-6 and H-7), 1.56 (3H, s, H-17), 1.53 (1H, m, H-3'), 1.27 (2H, m,
H-2'), 1.18 (3H, s, H-18), 1.07 (3H, d, J = 6.8 Hz, H-16), 0.89 (3H, d, J = 6.4 Hz, H-4'), 0.89 (3H, d, J
= 6.4, H-5'), 0.75 (3H, s, H-20), 13C-NMR (CDCl3): 37.63 (C-1), 19.98 (C-2), 37.89 (C-3), 44.28
(C-4), 53.93 (C-5), 21.25 (C-6), 37.62 (C-7), 127.18 (C-8), 139.42 (C-9), 39.95 (C-10), 26.12 (C-11),
34.71 (C-12), 31.75 (C-13), 41.36 (C-14), 170.88 (C-15), 19.89 (C-16), 20.14 (C-17), 28.86 (C-18),
178.55 (C-19), 18.14 (C-20), 51.51 (OCH3); Quinone: 180.69, 187.36 (C-1 and C-4), 149.00 (C-2),
141.28 (C-3), 137.32, 143.21 (C-4a, C-8a), 23.22, 22.71 (C-5 and C-8), 21.44, 21.44 (C-6 and C-7),
22.28 (C-1'), 38.13 (C-2'), 28.66 (C-3'), 22.84 (C-4'), 22.84 (C-5'); molecular formula: C36H52O6
(580.3764). m/z 581.3832 [M+H]+, error: 0.65 ppm with the empirical formula C36H53O6; m/z
598.4102 [M+NH4]+, error: 0.13 ppm with the empirical formula C36H56NO6.
3.3. Cell Culture
Human lung fibroblasts MRC-5 (ATCC CCL-171) were grown in minimum essential Eagle
medium (MEM) with Earles’s salts and 2 mM L-glutamine (Sigma-Aldrich Co.). Human epithelial
gastric cells AGS (ATCC CRL-1739) were grown in Ham F-12 medium containing 1 mM
L-glutamine. Both media were supplemented with 1.5 g/L sodium bicarbonate (Sigma-Aldrich Co.),
10% heat-inactivated fetal bovine serum (FBS), 100 IU/mL penicillin and 100 µg/mL streptomycin.
Both cell lines were grown as monolayers in a humidified incubator with 5% CO2 in air at 37 °C.
Culture media, antibiotics and FBS were obtained from Invitrogen Corp.
3.4. Cytotoxicity Assay
Cytotoxicity values were required as a reference to determine the working concentrations in the
subsequent experiments. Briefly, confluent cultures of MRC-5 and AGS cells were treated during 24 h
with medium containing the compounds at concentrations ranging from 0 up to 1,000 µM. The
products were first dissolved in DMSO (1% final concentration) and then in the corresponding culture
medium supplemented with 2% FBS. Untreated cells were used as controls. Cell viability was
determined at the end of the incubation by means of the neutral red uptake (NRU) assay [27].
Lansoprazole (Sigma-Aldrich Co., min. 98% by TLC) was used as reference compound.
3.5. MRC-5 Fibroblast Proliferation Assay
One day after seeding, cells were treated during 4 days with medium supplemented with 10% FBS
and the studied compounds at concentrations ranging from 1/64 up to 1/2 of the respective IC50 values.
Untreated cells were used as controls. Cell viability was determined at the end of the incubation by
means of the NRU assay [27].

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3.6. Sodium Taurocholate-Induced Damage to AGS Cells
The effect of sodium taurocholate (NaT) on cell viability was determined according to [15]. Briefly,
one day post-confluent AGS cells were incubated during 60 min with the compounds at 1/2, 1/4 and
1/8 of the respective IC50 values. Then, 10 mM NaT was added to all wells for 30 min. Untreated cells
were used as controls. Sucralfate (4 mg/mL; 580 µM) was used as reference compound (Sigma-Aldrich
Co., min. 30% sucrose octasulfate by HPLC). After incubation, the NRU assay was carried out [27].
3.7. Determination of Prostaglandin E2 (PGE2) Content
One day after confluence, AGS cells were treated for 1 h with the compounds at 1/2 and 1/4 of the
respective IC50 values. A control with medium only was included. Indomethacin (100 μM) was used as
standard inhibitor of prostaglandin synthesis (Sigma-Aldrich Co., min. 99% by TLC). After incubation,
PGE2 content was determined by means of a specific enzyme immunoassay kit (R & D Systems
KGE004B, Minneapolis, MN, USA) and values were calculated according to the manufacturer
instructions. Results are expressed as pg/well.
3.8. Determination of Cellular Reduced Glutathione (GSH) Content
One day after confluence, AGS cells were incubated during 4 h with the compounds at 1/4 and 1/8
of their respective IC50 values. Untreated cells were used as controls. The GSH synthesis stimulant
N-acetyl-L-cysteine (750 µM) was used as reference substance (Sigma-Aldrich Co., ≥ 90% by HPLC).
After incubation, the GSH content was determined using a colorimetric kit (BioAssays Systems,
Hayward, CA, USA). Results are expressed as nanomol of soluble reduced sulfhydryls/106 cells.
3.9. Inhibition of Lipoperoxidation in Erythrocyte Membranes
The inhibition of lipid peroxidation was determined using human erythrocyte membranes [28]. The
compounds were tested at 100 µg/mL. Catechin was used as the reference compound (Sigma-Aldrich
Co., min 98% by TLC).
3.10. Statistical Analysis
Results were expressed as mean values ± SD. Experiments with MRC-5 and AGS cells were carried
out three times using different cell preparations. Each concentration was tested in quadruplicate.
Statistical differences between different treatments and their respective control were determined by
one-way analysis of variance (ANOVA) followed by the Dunnett’s multiple comparison test. The level
of significance was set at p < 0.05 and p < 0.01. All statistical analyses were carried out using the
software SPSS 12.0 for Windows.
4. Conclusions
The aim of this work was to synthesize new diterpenylquinones, to assess the possible mechanisms
of the gastroprotective effects of the hybrid compounds using cell cultures and to establish some
structure-activity trends. The hybrid compounds displayed activities different from those shown by the

Molecules 2013, 18
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starting compounds, supporting the potential of this approach in the search for new bioactive
molecules. These effects were modulated by selective modification in the terpene or quinone moieties
of the hybrids. The changes in effect associated to the stereochemistry of the diterpenes (i.e., a more
flexible configuration for diterpenes with a 8(17) double bond or the 17-β-dihydroderivative vs. the
Δ8(9) isomer) or with modifications in the planar configuration of the napththoquinones, suggest that
the different mechanisms involved might include interactions with active sites of enzymes and/or
receptors. Molecular modeling can be a useful tool for better understanding the clues behind the
observed effects.
Acknowledgments
Financial support by FONDECYT (Project N 1110054) is kindly acknowledged. I.B. is grateful to
CONICYT for a doctoral grant (NAC-Doctorado N 21120593). We are indebted to Irene Manriquez
for her skilful technical work.
Conflicts of Interest
The authors declare no conflict of interest.
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Sample Availability: Samples of the starting products are available from the authors.
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