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24
induced silencing complex (RISC). In addition, a reduction
in negative charge occurs with several of these polyanionic
siRNAs, which may be assisting the siRNA favourably. Further-
more, placing cholesterol at the central region also functions
efficiency, and may offer a relatively new method to install
cholesterol small molecule centrally within siRNAs. This could
have important use in liposome or other related delivery
25
systems. For example, cholesterol-labeled siRNAs for liposome
packaging are oen labelled at one end of the siRNA. An siRNA
labelled with a cholesterol moiety at the central region could
potentially offer altered and improved packaging into lipo-
somes and/or lipid nanoparticles. Similarly, by altering the
hydrophobicity and positive charge of the various linkers used
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