Chemical Communications p. 2413 - 2414 (1996)
Update date:2022-08-24
Topics:
Jones, Nia E.
England, Paul A.
Rouch, Duncan A.
Wong, Luet-Lok
The regioselectivity of the catalytic hydroxylation of phenylcyclohexane 1 by cytochrome P450cam can be altered dramatically by site-directed mutagenesis: the Y96F single site mutant gives 81% cis-3-phenylcyclohexanol 2, with 22% enantiomeric excess (ee), while the Y96F-V247A double mutant gives 97% 2 with 42% ee and the Y96F-V247L double mutant gives 83% trans-4-phenylcyclohexanol 4.
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