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substrates: S69, G70, V71 and Y74 in AK and V71 in CK, which are
located on the GS region (see Fig. 1). In this work, we showed through
Ala scanning mutagenesis that in Arenicola MiTK, K67, T70 and V71
are associated with binding of taurocyamine. It should be noted that
V71 is relevant in the above-mentioned enzymes, AK, CK and MiTK,
suggesting a common role in binding guanidino substrates among
various phosphagen kinases. In Arenicola MiTK, residue 71 also
appears to be associated with the ability to distinguish among various
guanidino substrates, as the replacement of V71 by A71 caused a
dramatic change in enzyme activity, shifting the protein from a TK to
behaving like a GK.
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tubeworm Riftia pachyptila. Cloning and expression of cytoplasmic and mito-
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Acknowledgement
This work was supported by Grants-in-Aid for Scientific Research
in Japan to TS (17570062 and 20570072). We thank Dr. W. Ross
Ellington of Florida State University for his helpful suggestions.
[21] W.R. Ellington, K. Roux, A.O. Pineda, Origin of octameric creatine kinases, FEBS
Lett. 425 (1998) 75–78.
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