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Med Chem Res (2013) 22:3119–3125
Evaluation of ITC analogs against R. solani
of dithiocarbamate derivatives, which were then treated with
hydrogen peroxide to yield corresponding ITC derivatives.
Structure–activity relationships for the newly synthesized
compounds against plant pathogenic fungi and bacteria were
evaluated and discussed. The results indicated that the
activity of ITCs against R. solani, in general, reduced when
the chain length was increased, while the activity of ITCs
against E. carotovora was generally intensive with
increasing chain length. The present study revealed that
some of the compounds exhibited promising antimicrobial
activity and could be used as an acceptable alternative to the
traditional synthetic fungicides for controlling R. solani and
E. carotovora.
In this experiment, the efficacy of ITCs against R. solani was
determined by growth rate method. On the basis of the
preparation experiment, appropriate concentrations (lg/mL)
of ITCs were determined, and the blank flat and the flat with
the only solvent were used as the control. After rejuvenation
re-identification, fungus cake of R. solani (diameter 5 mm)
was vaccinated in the toxic flat, and cultured in a constant
temperature incubator at 24–28 °C. The percentage of rela-
tive inhibition of ITCs against fungus was determined by the
comparing colonial diameter after the culture.
Evaluation of ITC analogs against Envinia carotovora
Acknowledgments This work was supported by the Open Topics of
the XINJIANG Prodution & CORP Key Laboratory of Protection and
Utilization of Biological Resources in Tarim Basin (BRYB1104).
The synthesized compounds were tested for antimicrobial
activity against E. carotovora by the turbidimetric method.
9
An aliquot (0.1 mL) of the bacterial suspension (10 CFU/
mL) was incorporated into a conical flask (35 mL) con-
taining 10 mL of lysogeny broth (LB), which contained
with ITCs at different concentrations (lg/mL) using
DMSO as solvent. DMSO concentrations in both control
and assay were below 0.1 % (v/v). The mixtures were
inoculated at 30 °C for 12 h. The turbidity of the bacteria
solution was examined with the turbidimetric method using
an Agilent 8453 UV–Visible spectrophotometer. A sample
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The half maximal effective concentration (EC ) values
5
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˜
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A series of aliphatic ITCs were prepared by reacting ali-
phatic amines with carbon disulfide resulting in precipitation
1
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