Bioscience, Biotechnology and Biochemistry p. 1721 - 1729 (1999)
Update date:2022-08-16
Topics:
Xie, Sheng-Xue
Ogawa, Jun
Shimizu, Sakayu
An NAD+-dependent alcohol dehydrogenase was purified to homogeneity from Nocardia fusca AKU 2123. The enzyme catalyzed (S)-specific oxidation of 3-pentyn-2-ol (PYOH), i.e., part of the stereoinversion reaction for the production of (R)-PYOH, which is a valuable chiral building block for pharmaceuticals, from the racemate. The enzyme used a broad variety of secondary alcohols including alkyl alcohols, alkenyl alcohols, acetylenic alcohols, and aromatic alcohols as substrates. The oxidation was (S)-isomer specific in every case. The Km and Vmax for (S)-PYOH and (S)-2-hexanol oxidation were 1.6 mM and 53 μmol/min/mg, and 0.33 mM and 130/μmol/min/mg, respectively. The enzyme also catalyzed stereoselective reduction of carbonyl compounds. (S)-2-Hexanol and ethyl (R)-4-chloro-3-hydroxybutanoate in high optical purity were produced from 2-hexanone and ethyl 4-chloro-3-oxobutanoate by the purified enzyme, respectively. The Km and Vmax for 2-hexanone reduction were 2.5 mM and 260 μmol/min/mg. The enzyme has a relative molecular mass of 150,000 and consists of four identical subunits. The NH2-terminal amino acid sequence of the enzyme shows similarity with those of the carbonyl reductase from Rhodococcus erythropolis and phenylacetaldehyde reductase from Corynebacterium sp.
View MoreLishui Nanming Chemical Co., Ltd
Contact:+86-0578-2134101,2697830
Address:No.19 Tongji Road Shuige Industrial zone
YanCheng LongShen Chemical Co.,Ltd.
Contact:+86-515-86668866
Address:No.13,Weiyi Road,Funing Aoyang Industrial Park
Hangzhou GreenCo Science & Technology Co., Ltd.
Contact:86-571-88257303
Address:1713 Room,Jingui Building,Gudun Road,Xihu District,Hangzhou,China
Contact:+86-575-82733999 0575-82732999
Address:hangzhou gulf fine chemical zone,shangyu city,zhejiang province
Contact:0792-8228321
Address:10TH Floor No.121 binjiang Road Xunyang District
Doi:10.1063/1.447052
(1984)Doi:10.1021/ja005733y
(2001)Doi:10.1134/S1070363210010238
(2010)Doi:10.1016/j.farmac.2004.05.002
(2004)Doi:10.1016/S0040-4020(00)00974-1
(2000)Doi:10.1039/c9tc04680a
(2019)