Fluorescein Chemiluminescence-Delay Method for the Determination of Ultratrace Amounts of Copper(II)

Article abstract of DOI:10.1246/cl.1992.887

A delayed chemiluminescence (CL) was observed in the copper(II)-catalyzed oxidation of cysteamine with oxygen in the presence of fluorescein (FL) and horseradish peroxidase.The delayed CL reaction of FL was applied to the determination of Cu(II).The delay time was correlated linearly with Cu(II) concentration over the range from 5.0 * 10^-9 M to 1.0 * 10^-6 M.