6
K.-M. KIM ET AL.
required in enzymatic biosynthesis. Moreover, the sys-
tem is much superior to chemical synthesis because of
the absence of hazardous chemicals.
Funding
This research was supported by Basic Science Research
Program through the National Research Foundation of Korea
(NRF) funded by the Ministry of Science, ICT & Future
Planning (2017R1C1B5018228) to Prof. Seung-Young Kim.
The research was also financially supported by the Ministry
of Trade, Industry and Energy (MOTIE), Korea under
“Regional Specialized Industry Development Program” super-
vised by the Korea Institute for Advancement of Technology
(KIAT) (Grant No: R0006496).
Four derivatives of daidzein were obtained in this
study. All of the molecules were structurally character-
ized. The mass and NMR spectra were validated by
comparing with previously published data. One of the
molecules, 40-ethoxy-daidzein-7-O-b-(600-O-succinyl)-D-
glucoside, was a new derivative isolated from the bio-
transformation reaction mixture of resting cells of B.
amyloliquefaciens and is a novel candidate for the
chemical library. The physiochemical and biological
activities of these molecules will be studied in fur-
ther research.
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genes that play
bioconversion.
a
crucial role in daidzein
Though glycerin is utilized by cells as a carbon
source, recently, very high concentrations of glycerin
(above 90 g/L; 9%) were found to be inhibitory for the
growth of Clostridium butyricum (Szymanowska-
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biotransformation capabilities of microbial cells was
reported previously. Thus, this subject could be of
interest among microbiologists to identify potential
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gene expression.
In conclusion, the method using resting cells in buf-
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single precursor in a short period of time. Further tran-
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Disclosure statement
ment and management inputs.
J Sci Food Agric.
No potential conflict of interest was reported by the authors.
97:3342–3347.