STRESS-INDUCED FORMATION OF ECHINATIN AND A METABOLITE, 5'-PRENYL-LICODIONE, IN CULTURED GLYCYRRHIZA ECHINATA CELLS

Article abstract of DOI:10.1016/S0031-9422(00)83745-0

Key Word Index - Glycyrrhiza echinata; Leguminosae; cell culture; induction; stress metabolites; yeast extract; flavonoids; retrochalcone; echinatin; 5'-prenyl-licodione.The production of a retrochalcone, echinatin, by isoflavone-rich Glycyrrhiza echinata (M-2) cultured cells was stimulated by the addition of yeast extract or calcium alginate beads to the culture medium.Combined addition of yeast extract and cycloheximide suppressed the formation of retrochalcone, suggesting de novo synthesis.A new metabolite was isolated from the induced cells and its structure was determined to be 1-<2,4-dihydroxy-5-(3-methyl-2-butenyl)phenyl>-3-(4-hydroxyphenyl)-1,3-propanedione (5'-prenyl-licodione).