Determination of the Carbamate Herbicide Propham by Synchronous Derivative Spectrofluorometry following Fluorescamine Fluorogenic Labeling

Article abstract of DOI:10.1021/ac00292a018

Three methods for the determination of propham by fluorogenic labeling with fluorescamine and synchronous derivative spectrofluorometry are described and discussed.The herbicide is hydrolyzed in 1 M NaOH at 100 deg C for 40 min, and the liberated aniline reacts instantaneously wih fluorescamine in an aqueous buffer solution (pH 4.0).Reaction rates, fluorescence phenomena, and synchronous derivative parameters are investigated.To enhance sensitivity, the analysis of the fluorescamine derivative is used following extraction in ethyl acetate.Linear analytical curves are obtained between 24 and 2000 ng/mL.The minimum detectable quantity is estimated to be 7.2 ng/mL.Wellwater samples containing low concentrations of carbamate are also analyzed.