10.1016/j.ejmech.2013.11.020
The research focuses on the design and synthesis of thiazole derivatives as potent FabH inhibitors with antibacterial activity. The study aimed to develop new antibacterial agents by targeting the fatty acid biosynthetic pathway, specifically the enzyme FabH. Two series of compounds, A (4ae4g) and B (5ae5g), were synthesized by forming an amine bond between aromatic acids and 4-phenylthiazol-2-amine or 4-(4-bromophenyl)thiazol-2-amine. These thiazole derivatives were evaluated for their antibacterial activity against four bacterial strains using the MTT assay, with minimum inhibitory concentration (MIC) values ranging from 1.56 mg/mL to 100 mg/mL. The compounds also showed FabH inhibition ability with IC50 values ranging from 5.8 mM to 48.1 mM. The research involved the synthesis of key intermediates, the formation of amide bonds, and the evaluation of antibacterial activity. Docking simulations and 3D-QSAR studies were conducted to understand the binding mode and structure-activity relationships. The analyses included elemental analysis, NMR, MS, and enzyme assays to determine the IC50 values. The experiments used various reagents, such as aluminum chloride, bromine, thiourea, and acetyl chloride, and involved techniques like thin-layer chromatography, melting point determination, and ESI mass spectrometry. The study concluded that compound 5f exhibited the best antibacterial and E. coli FabH inhibitory activity, suggesting its potential as an antibacterial agent.