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Metabolic profiling

the systematic study of the unique chemical fingerprints that specific cellular processes leave behind

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In vitro Synthetic route Mass spectrometry (MS) HPLC (high-performance liquid chromatography) in vivo Purification Deuterium Source Metabolism Kinetic Isotope Effect (KIE) Dithiocarbamates Gas Chromatography-Mass Spectrometry (GC-MS) Enzyme activity assay Reaction yield Metabolites Metabolic pathway Liquid chromatography-mass spectrometry (LC-MS) Deuterated solvent NMR spectroscopy (Nuclear Magnetic Resonance) Stability testing Deuterium (D)