Design the sensor mechanism. In this case, you want fluorescence to be quenched (off-state) in the absence of Fe3? ions and turned on (on-state) when Fe3? ions are present. This can be achieved through various mechanisms, such as the F?rster resonance energy transfer (FRET) or through structural changes in the derivative upon binding to Fe3? ions.
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6-anilino-quinazolino[4,3-b]quinazolin-8-one
