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5704-04-1

Basic Information
CAS No.: 5704-04-1
Name: Glycine,N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-
Article Data: 4
Cas Database
Molecular Structure:
Molecular Structure of 5704-04-1 (Glycine,N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-)
Formula: C6H13NO5
Molecular Weight: 179.173
Synonyms: N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine;N-[Tris(hydroxymethyl)methyl]glycine;NSC 369995;
EINECS: 227-193-6
Density: 1.449 g/cm3
Melting Point: 186-188 °C (dec.)
Boiling Point: 500.9 °C at 760 mmHg
Flash Point: 256.7 °C
Solubility: water: 4 M at 20 °C, clear, colorless
Appearance: White/clear crystalline powder
Hazard Symbols: IrritantXi
Risk Codes: 36/37/38
Safety: 26-36-36/37/39
Transport Information: N/A
PSA: 110.02000
LogP: -2.23280
Synthetic route

sodium [99Tc]pertechnetate

139-13-9

nitrilotriacetic acid

5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

(99m)Tc(HYNIC-Lys(OMe)-NIC)(tricine)

Conditions
ConditionsYield
With hydrogenchloride; tin(ll) chloride In water at 100℃; for 0.166667h; pH=5.0; Succinate buffer;95%

1-(N-(6-(2-(2-sulfonatobenzaldehyde)hydrazono)nicotinyl))-6-(nicotinoyl) hexanediamine

sodium [99Tc]pertechnetate

5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

(99m)Tc(HYNIC-HD-NIC)(tricine)

Conditions
ConditionsYield
With hydrogenchloride; tin(ll) chloride In ethanol; water at 100℃; for 0.166667h; pH=5.0;95%

HYNIC-Lys(OMe)-His

sodium [99Tc]pertechnetate

5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

(99m)Tc(HYNIC-Lys(OMe)-His)(tricine)

Conditions
ConditionsYield
With hydrogenchloride; tin(ll) chloride In water at 100℃; for 0.25h; pH=5.0; Succinate buffer;95%
5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

27668-52-6

octadecyldimethyl[3-(trimethoxysilyl)propyl]ammonium chloride

disodium 7-(2-((carboxymethyl)amino)-3-hydroxy-2-(hydroxymethyl)propoxy)-7-(3-(dimethyl(octadecyl)ammonio)propyl)-4,4,10,10-tetrakis(hydroxymethyl)-6,8-dioxa-3,11-diaza-7-silatridecanedioate chloride

Conditions
ConditionsYield
Stage #1: N-[tris(hydroxymethyl)methyl]glycine; octadecyldimethyl[3-(trimethoxysilyl)propyl]ammonium chloride In N,N-dimethyl-formamide at 145℃;
Stage #2: With sodium methylate In N,N-dimethyl-formamide
89%

[Re(carbonyl)3(water)3]Br

5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

121-44-8

triethylamine

[HNEt3][Re(CO)3(κ3-NH2,O,CO2-tricine)][Re(CO)3(κ3-NH2,OH,CO2-tricine)]*2H2O

Conditions
ConditionsYield
In water Re(CO)3(H2O)3Br (0.371 mmol), tricine (0.0445 mmol), NEt3 (1.12 mmol) inH2O refluxed (0.5 h); solvent removed; washed with acetone; elem. anal.;88%
5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

3H3N*3H(1+)*[CrMo6O18(OH)6](3-)

3H3N*3H(1+)*[HOOCCH2NHC(CH2O)3]CrMo6O18(OH)3(3-)

Conditions
ConditionsYield
In water at 130℃; for 12h;88%
5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

cobalt(II) nitrate

2382-96-9

2-sulfanyl-1,3-benzoxazole

[Co[(HOCH2)3CNCH2COO][C7H4NO(S)]2](2-)*2H(1+)=H2[Co[(HOCH2)3CNCH2COO][C7H4NO(S)]2]

Conditions
ConditionsYield
With KOH In ethanol Co-salt in H2O added slowly to tricine in EtOH with stirring, mercapto-comp. in EtOH added dropwise with stirring, pH ajusted to 8 with 0.1 M KOH, refluxed for 5 h; evapd., cooled, filtered, washed with H2O and EtOH, dried in vac. over P4H10, elem. anal.;55%
5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

149-30-4

2-Mercaptobenzothiazole

nickel(II) nitrate

[Ni[(HOCH2)3CNCH2COO][C7H4NS(S)]2(H2O)2](2-)*2H(1+)=H2[Ni[(HOCH2)3CNCH2COO][C7H4NS(S)]2(H2O)2]

Conditions
ConditionsYield
With KOH In ethanol Ni-salt in H2O added slowly to tricine in EtOH with stirring, mercapto-comp. in EtOH added dropwise with stirring, pH ajusted to 8 with 0.1 M KOH, refluxed for 5 h; evapd., cooled, filtered, washed with H2O and EtOH, dried in vac. over P4H10, elem. anal.;53%
5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

copper(II) nitrate

134469-07-1

Benzimidazol-2-thiol

[Cu[(HOCH2)3CNCH2COO][C7H5N2(S)]2](2-)*2H(1+)=H2[Cu[(HOCH2)3CNCH2COO][C7H5N2(S)]2]

Conditions
ConditionsYield
With KOH In ethanol Cu-salt in H2O added slowly to tricine in EtOH with stirring, mercapto-comp. in EtOH added dropwise with stirring, pH ajusted to 8 with 0.1 M KOH, refluxed for 5 h; evapd., cooled, filtered, washed with H2O and EtOH, dried in vac. over P4H10, elem. anal.;52%
148-24-3

8-quinolinol

nickel(II) nitrate hexahydrate

5704-04-1

N-[tris(hydroxymethyl)methyl]glycine

H(1+)*Ni((HOCH2)3CNCH2CO2)(C5H3NC4H3O)(H2O)2(1-)*H2O=H[Ni((HOCH2)3CNCH2CO2)(C5H3NC4H3O)(H2O)2]H2O

Conditions
ConditionsYield
With KOH In ethanol; water soln. of nitrate salt in water added slowly to soln. of tricine in C2H5OH with stirring, soln. of hydroxyquinoline in C2H5OH added dropwise withstirring, adjusting pH to 8 by aq. KOH, mixt. refluxed for 5 h; mixt. evapd., cooled, ternary complex filtered off, washed (H2O, C2H5OH), dried (vac., P4O10); elem. anal.;51%
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Chemistry

IUPAC Name:   2-[[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
Synonyms:  N-[1,3-Dihydroxy-2-(hydroxymethyl)-2-propanyl]glycine ; N-[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]glycine ; N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine ; Glycine, N-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)- ; N-(2-hydroxy-1,1-bis[hydroxymethyl]ethyl)glycine ; N-(tri(hydroxymethyl)methyl)glycine ; N-(Trishydroxymethylmethyl)glycine
The Molecular Formula of  Tricine (5704-04-1):C6H13NO5
The Molecular Weight of Tricine (5704-04-1):179.1711 g/mol
The Molecular Structure of  Tricine (5704-04-1):
Index of Refraction: 1.553
Molar Refractivity: 39.57 cm3
Molar Volume: 123.5 cm3 
Surface Tension: 76.8 dyne/cm
Density: 1.449 g/cm3
Flash Point: 256.7 °C
Enthalpy of Vaporization: 88.63 kJ/mol
Boiling Point: 500.9 °C at 760 mmHg
Vapour Pressure: 3.92E-12 mmHg at 25°C 

Uses

1.  Tricine (5704-04-1) is an organic compound that is used in buffer solution.
2.It  is also used in resuspension of cell pellet.

Safety Profile

Hazard Codes: IrritantXi
Risk Statements: 36/37/38 
R36/37/38:Irritating to eyes, respiratory system and skin.
Safety Statements :26-36-36/37/39
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. 
S36:Wear suitable protective clothing. 
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection.
WGK Germany: 3
F: 3