102573-69-3

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 94189-75-0 N³-benzoyl thymidine 
• 130867-58-2 Methoxy-acetic acid (2R,3S,5R)-5-(3-benzoyl-5-methyl-2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-yl)-2-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-tetrahydro-furan-3-yl ester 
• 146952-74-1 5'-O-triethylsilylthymidine 
• 50-89-5 thymidine 
• 98-88-4 benzoyl chloride 
• 40615-39-2 5'-O-(4-4'-dimethoxytrityl)thymidine 
• 126144-60-3 3'-O-trimethylsilyl-5'-O-dimethoxytrityl-thymidine 
• 130867-57-1 5’-O-dimethoxytrityl-3’-O-methoxyacetylthymidine 

Downstream Products

• 130867-59-3 5'-O-dimethoxytrityl-3'-O-methylthiomethyl-N³-benzoyl-thymidine 
• 130867-60-6 5'-O-dimethoxytrityl-3'-O-(4-pentenyloxymethyl)-N³-benzoyl-thymidine 
• 131853-35-5 5'-O-(4,4'-dimethoxytrityl)-N³-benzoylthymidine 3'-N,N-diisopropylphosphonoamidate 
• 130983-93-6 C₆₃H₅₉N₄O₁₆P 
• 102538-11-4 methyl 5'-O-dimethoxytrityl-N³-benzoylthymidin-3'-yl N³,3'-O-dibenzoylthymidin-5'-yl phosphate 
• 130867-62-8 3-Benzoyl-1-[(2R,4S,5R)-5-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-4-(2,5-dioxo-pyrrolidin-1-ylmethoxy)-tetrahydro-furan-2-yl]-5-methyl-1H-pyrimidine-2,4-dione 
• 130867-65-1 C₃₈H₄₀N₄O₁₄ 
• 130867-68-4 C₅₆H₅₄N₄O₁₄ 
• 130867-64-0 C₅₉H₅₈N₄O₁₆ 
• 130887-31-9 C₆₅H₇₁N₆O₁₅P 
• 133510-59-5 C₆₁H₆₄N₆O₂₂ 
• 133510-52-8 3-Benzoyl-1-((2R,4S,5R)-5-hydroxymethyl-4-methylsulfanylmethoxy-tetrahydro-furan-2-yl)-5-methyl-1H-pyrimidine-2,4-dione 
• 133510-54-0 3-Benzoyl-1-((2R,4S,5R)-5-hydroxymethyl-4-pent-4-enyloxymethoxy-tetrahydro-furan-2-yl)-5-methyl-1H-pyrimidine-2,4-dione 
• 133510-53-9 5'-O-levulinoyl-3'-O-methylthiomethyl-N³-benzoyl-thymidine 

Relevant academic research and scientific papers

PHOTORESPONSIVE DEOXYRIBONUCLEOSIDE TRIPHOSPHATE

PROBLEM TO BE SOLVED: To provide photoresponsive deoxyribonucleoside triphosphate in which the initiation of the amplification reaction of a target nucleic acid can be controlled by light irradiation, and to provide its production precursor. SOLUTION: The above problem is solved by the compound represented by the general formula (1) (where, R1 and R2 each independently represent an alkyl group having 1 to 8 carbon atoms; R3 and R4 independently represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms, or a phenyl group; R5 represents a hydrogen atom or a group represented by the formula (7) [-P(=O)(OH)-O-P(=O)(OH)-O-P(=O)(OH)2(7)]; Y represents a hydrogen atom or a hydroxy group; and X represents a nucleobase). SELECTED DRAWING: None COPYRIGHT: (C)2022,JPOandINPIT

METHOD OF SYNTHESIZING SINGLE-STRANDED NUCLEOTIDE SEQUENCE, BLOCKED NUCLEOSIDE TRIPHOSPHATES AND RELATED METHODS

There is provided a method of synthesizing a single-stranded nucleotide sequence, the method comprising adding a blocked nucleoside triphosphate to an initiator nucleotide sequence to incorporate a corresponding blocked nucleotide thereto in the presence of a polymerase, wherein the blocked nucleoside triphosphate has one of the general formulae (I), (II), (III), (IV), (V) and (VI).

Design and synthesis of dephosphono dna analogues containing 1,2,3-triazole linker and their uv-melting studies with DNA/RNA

This article describes the synthesis of 3/5 linked 1,2,3-triazolyl dithymidine derivatives, their incorporation into oligonucleotides, and evaluation of their thermal stabilities toward complementary DNA/RNA. Copyright Taylor and Francis Group, LLC.

Synthesis of thymidine dimers from 5′-O-aminothymidine

The synthesis of modified oligonucleotides is of great importance for various therapeutic and diagnostic applications. The facile secondary structure formation of N-oxyamide-linked peptide analogues and the high nucleophilicity of the aminooxy function prompted us to prepare O-amino nucleoside derived dinucleosides. Herein, the efficient synthesis of three novel thymidine dimers with N-oxyamide, oxime and oxyamine linkages via a convergent approach from a common 5-O-aminothymidine is reported. Georg Thieme Verlag Stuttgart · New York.

Synthesis of oligodeoxynucleotides using fully protected Deoxynucleoside 3c-Phosphoramidite building blocks and base recognition of Oligodeoxynucleotides incorporating N3-Cyano-Ethylthymine

Oligodeoxynucleotide (ODN) synthesis, which avoids the formation of side products, is of great importance to biochemistry-based technology development. One side reaction of ODN synthesis is the cyanoethylation of the nucleobases. We suppressed this reaction by synthesizing ODNs using fully protected deoxynucleoside 3c-phosphoramidite building blocks, where the remaining reactive nucleobase residues were completely protected with acyl-, diacyl-, and acyl-oxyethylene-type groups. The detailed analysis of cyanoethylation at the nucleobase site showed that N3-protection of the thymine base efficiently suppressed the Michael addition of acrylonitrile. An ODN incorporating N3-cyanoethylthymine was synthesized using the phosphoramidite method, and primer extension reactions involving this ODN template were examined. As a result, the modified thymine produced has been proven to serve as a chain terminator.

Simple and efficient solution-phase synthesis of oligonucleotides using extractive work-up

A solution-phase synthesis protocol amenable to scale-up was developed for the preparation of oligonucleotides employing phosphoramidite chemistry and DMTr/iBu/Bz-protected monomers. Isolation of intermediates was accomplished by means of extractions as the only purification tool. The potential of the method is demonstrated with the synthesis of a hexameric DNA fragment in high yield and purity.

An efficient approach to the synthesis of thymidine derivatives containing phosphate-isosteric methylene acetal linkages

Iodonium ion promoted condensation of properly protected 3′-O-methylthiomethyl or 3′-O-(4-penten-1-oxymethyl)-thymidine with 3′-O-methoxyacetyl-thymidine, was explored. A judicious choice of the iodoinium source and protecting groups led to an efficient preparation of thymidine dimers having internucleosidic-3′5′)-methylene bonds. The latter procedure was utilized towards the synthesis, in solution and on a solid support, of DNA-fragments containing one or more T-CII2-T dimers. Further, 5′-O-methylthiomethyl-3′-O-methoxyacetyl-N 3-benzoyl-thymidine proved to be a suitable donor for the introduction of 5′-O-methylene acetal-linkage s between 2,3,4,6-tetra-Obenzyl-D-glucose, benzyl N-benzyloxycarbonyl-L-serine and dibenzyl phosphate.

SYNTHESIS OF OLIGODEOXYNUCLEOTIDES CONTAINING THYMIDINES LINKED VIA AN INTERNUCLEOSIDIC-(3'-5')-METHYLENE BOND

Condensation of N3-benzoyl-5'-O-dimethoxytrityl-3'-O-(4-penten-1-oxomethyl)-thymidine with N3-benzoyl-3'-O-methoxyacetyl-thymidine, in the presence of N-iodosuccinimide, afforded a fully-protected thymidine dimer having an internucle

A NEW CLASS OF CONDENSING REAGENTS FOR RAPID INTERNUCLEOTIDE BOND FORMATION IN THE PHOSPHOTRIESTER APPROACH AND PREPARATION OF N3-BENZOYLTHYMIDINE AS A KEY INTERMEDIATE IN OLIGODEOXYRIBONUCLEOTIDE SYNTHESIS

Rapid internucleotide bond formation in the phosphotriester approach has been achieved in high yield by use of bis(2,4,6-trihalophenyl)phosphorochloridates (TCP and TBP) as new condensing reagents and the benzoyl group as the N3-imide protecting group of thymidine.