Antiviral activity of the MEK-inhibitor U0126 (cas 109511-58-2) against pandemic H1N1v and highly pathogenic avian influenza virus in vitro and in vivo
-
Add time:07/20/2019 Source:sciencedirect.com
The emergence of the 2009 H1N1 pandemic swine influenza A virus is a good example of how this viral infection can impact health systems around the world in a very short time. The continuous zoonotic circulation and reassortment potential of influenza A viruses (IAV) in nature represents an enormous public health threat to humans. Beside vaccination antivirals are needed to efficiently control spreading of the disease. In the present work we investigated whether the MEK inhibitor U0126 (cas 109511-58-2), targeting the intracellular Raf/MEK/ERK signaling pathway, is able to suppress propagation of the 2009 pandemic IV H1N1v (v = variant) as well as highly pathogenic avian influenza viruses (HPAIV) in cell culture and also in vivo in the mouse lung. U0126 showed antiviral activity in cell culture against all tested IAV strains including oseltamivir resistant variants. Furthermore, we were able to demonstrate that treatment of mice with U0126 via the aerosol route led to (i) inhibition of MEK activation in the lung (ii) reduction of progeny IAV titers compared to untreated controls (iii) protection of IAV infected mice against a 100× lethal viral challenge. Moreover, no adverse effects of U0126 were found in cell culture or in the mouse. Thus, we conclude that U0126, by inhibiting the cellular target MEK, has an antiviral potential not only in vitro in cell culture, but also in vivo in the mouse model.
We also recommend Trading Suppliers and Manufacturers of U0126 (cas 109511-58-2). Pls Click Website Link as below: cas 109511-58-2 suppliers
Prev:mTOR inhibitor temsirolimus and MEK1/2 inhibitor U0126 (cas 109511-58-2) promote chromosomal instability and cell type-dependent phenotype changes of glioblastoma cells
Next:Quantitative NMR analysis of Erk activity and inhibition by U0126 (cas 109511-58-2) in a panel of patient-derived colorectal cancer cell lines) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- MEK inhibitors: The chemistry and biological activity of U0126 (cas 109511-58-2), its analogs, and cyclization products07/28/2019
- Using U0126 (cas 109511-58-2) to dissect the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) cascade in the regulation of gene expression by endothelin-1 in cardiac myocytes07/27/2019
- Non-specific effects of the MEK inhibitors PD098,059 and U0126 (cas 109511-58-2) on glutamate release from hippocampal synaptosomes07/26/2019
- Prevention of LLC-PK1 cell overgrowth in a bioartificial renal tubule device using a MEK inhibitor, U0126 (cas 109511-58-2)07/25/2019
- Nociceptin and its metabolite attenuate U0126 (cas 109511-58-2)-induced memory impairment through a nociceptin opioid peptide (NOP) receptor-independent mechanism07/24/2019
- Effects of MEK-Specific Inhibitor U0126 (cas 109511-58-2) on the Conidial Germination, Appressorium Production, and Pathogenicity of Setosphaeria turcica07/23/2019
- Quantitative NMR analysis of Erk activity and inhibition by U0126 (cas 109511-58-2) in a panel of patient-derived colorectal cancer cell lines07/22/2019
- mTOR inhibitor temsirolimus and MEK1/2 inhibitor U0126 (cas 109511-58-2) promote chromosomal instability and cell type-dependent phenotype changes of glioblastoma cells07/21/2019
- U0126 (cas 109511-58-2) attenuates ischemia/reperfusion-induced apoptosis and autophagy in myocardium through MEK/ERK/EGR-1 pathway07/19/2019