Rapid and simple determination of oxolinic acid and oxytetracycline in the shell of the blue mussel (Mytilus edulis) by high-performance liquid chromatography

Add time:07/20/2019    Source:sciencedirect.com

A simple procedure for the determination of oxolinic acid (OA) and oxytetracycline (OTC), two antibacterial agents, in the shell of the blue mussel (Mytilus edulis), using reversed-phase high-performance liquid chromatography is described. Liquid chromatography was performed on a 5-μm LiChroSpher 100 RP-18E column using acetonitrile and a 0.02 M orthophosphoric acid solution as the mobile phase, with ultraviolet detection. After roughly grinding the shell, drugs were extracted using a methanolic oxalic acid solution. Linearity and precision were checked over the concentration range 0.04–0.32 μg/g. Limits of detection of OA and OTC were 0.012 and 0.008 μg/g, respectively. Mean extraction recoveries of OA and OTC from mussel shell were 72.9 and 65.4%, respectively. To demonstrate the usefulness of the analytical procedure, an experimental study was performed in blue mussels exposed to the drugs for eight days.

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