The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein

Add time:07/22/2019    Source:sciencedirect.com

The STE4 and STE18 genes are required for haploid yeast cell mating. Sequencing of the cloned genes revealed that the STE4 polypeptide shows extensive homology to the β subunits of mammalian G proteins, while the STE18 polypeptide shows weak similarity to the γ subunit of transducin. Null mutations in either gene can suppress the haploid-specific cell-cycle arrest caused by mutations in the SCG1 gene (previously shown to encode a protein with similarity to the α subunit of G proteins). We propose that the products of the STE4 and STE18 genes comprise the β and γ subunits of a G protein complex coupled to the mating pheromone receptors. The genetic data suggest pheromone—receptor binding leads to the dissociation of the α subunit from βγ (as shown for mammalian G proteins), and the free βγ element initiates the pheromone response.

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