A stereoselective synthesis of 1α-(3′-Carboxypropyl)-4-androsten-17β-ol-3-one: Preparation of immunoreagents for quantification of testosterone by fluorescence polarization immunoassay
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Add time:07/14/2019 Source:sciencedirect.com
1α(3′-Carboxypropyl)-4-androsten-17β-ol-3-one (2), a novel hapten, was prepared from boldenone (3) via addition of 4-pentenyl magnesium bromide in the presence of CuBr, as a key step in >95% epimeric excess and 13.8% overall yield. Two immunogens (7,8) and three fluorescent tracers (11,18,19) were prepared from acid (2) for the development of an immunoassay for testosterone (1).
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