Chondroitin sulfate/dermatan sulfate from corb (Sciaena umbra) skin: Purification, structural analysis and anticoagulant effect

Add time:07/31/2019    Source:sciencedirect.com

In this study, chondroitin sulfate/dermatan sulfate was isolated and purified from the skin of corb (Sciaena umbra) (CSG) with a yield of 6.2%. Chemical and structural analysis showed that CSG consisted of high sulfate content 28.74% and an average molecular weight of 15.46 KDa. The separation of CSG by agarose-gel electrophoresis revealed the presence of DS and CS. Structural analysis of the purified CS/DS by means of SAX–HPLC after treatment with specific chondroitinases showed that this polymer was composed of nonsulfated disaccharide, monosulfated disaccharides and disulfated disaccharides in various percentages. The results also suggest that the percentage of CS and DS recovred in CSG were 24% and 76%, respectively. Anticoagulant activity in vitro was measured in plasma using classical anticoagulation tests: activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. The findings thus indicated that the purified CS/DS exhibits a remarkably high anticoagulant effect.

We also recommend Trading Suppliers and Manufacturers of (6S)-5,6,7,8-TETRAHYDRO-L-BIOPTERIN SULFATE (cas 109784-74-9). Pls Click Website Link as below: cas 109784-74-9 suppliers

Prev:Heparan sulfate in chronic kidney diseases: Exploring the role of 3-O-sulfation
Next:Phenotypic characterization and complementation analysis of Bacillus subtilis 6S RNA single and double deletion mutants)