Preparation of the methyl ester of hyaluronan and its enzymatic degradation

Add time:07/29/2019    Source:sciencedirect.com

A methyl ester of hyaluronan in which the carboxyl groups were fully esterified was prepared using trimethylsilyl diazomethane. This derivative, while not depolymerized by hyaluronan lyases or hyaluronan hydrolases, was a substrate for both chondroitin ACI lyase (EC 4.2.2.5) from Flavobacterium heparinum and chondroitin ACII lyase (EC 4.2.2.5) from Arthrobacter aurescens. The major product isolated in these depolymerization reactions was methyl α-l-threo-hex-4-enepyranosyluronate-(1→3)-2-acetamido-2-deoxy-α,β-d-glucopyranoside as determined by 1H NMR spectroscopy and MALDITOF mass spectrometry.

We also recommend Trading Suppliers and Manufacturers of methyl 2-acetamido-2-deoxy-3-O-(alpha-idopyranosyluronic acid)-4-O-sulfo-beta-galactopyranoside (cas 109914-81-0). Pls Click Website Link as below: cas 109914-81-0 suppliers

Prev:Synthesis of the methyl glycosides of a tri- and a tetra-saccharide related to heparin and heparan sulphate
Next:Chondroitin O-methyl ester: an unusual substrate for chondroitin AC lyase)