Inhibition of [3H]PONASTERONE A (cas 13408-56-5) binding by ecdysone agonists in the intact Sf-9 cell line

Add time:07/31/2019    Source:sciencedirect.com

Ecdysone agonists, including dibenzoylhydrazines, significantly inhibited the binding of [3H]ponasterone A ([3H]PoA) in intact Sf-9 cells (Spodoptera frugiperda). The amount of [3H]PoA binding varied in a concentration-dependent manner. According to the IC50, concentration at which there is 50% inhibition, the order of potency of typical ecdysone agonists is tebufenozide (RH-5992) > methoxyfenozide (RH-2485) > PoA > 20-hydroxyecdysone > cyasterone > RH-5849, makisterone A ≥ inokosterone > ecdysone. The ranking is consistent with that obtained from a cultured integument system of the rice stem borer Chilo suppressalis except for methoxyfenozide. Other compounds whose modes of action are different from that of ecdysteroids, for example respiration inhibitors, plant steroid hormones, and chitin synthesis inhibitors, did not inhibit the binding of [3H]PoA significantly. The mammalian hormones estradiol and diethylstilbestrol, and a secondary bile acid, lithocholic acid, significantly inhibited the binding of [3H]PoA at 25 μM. However, their binding activity in terms of pIC50 was either very low or not evaluated.

We also recommend Trading Suppliers and Manufacturers of PONASTERONE A (cas 13408-56-5). Pls Click Website Link as below: cas 13408-56-5 suppliers

Prev:The uptake and release of PONASTERONE A (cas 13408-56-5) by the Kc cell line of Drosophila melanogaster
Next:PONASTERONE A (cas 13408-56-5): A new ecdysteroid from the embyros and serum of brachyuran crustaceans)