Effect of fluorescence intensifiers on the fluorodensitometric determination of flavones and flavonols after detection with diphenylboric acid 2-aminoethyl ester

Add time:07/26/2019    Source:sciencedirect.com

The measurement of the fluorescence emitted by flavones and flavonols in situ on thin-layer chromatography plates can be improved by dipping the developed plates in appropriate solutions. The effect of different substances on the intensity and stability of the fluorescence of 14 flavone and 26 flavonol derivatives detected with diphenylboric acid 2-aminoethyl ester (Naturstoffreagens A, NA) is described. Silicone oil or paraffin is most appropriate for lipophilic flavonoids while the more hydrophilic polyethylene glycol 4000 is most appropriate for less lipophilic aglycones as well as glycosides. Authentic reference standards in appropriate concentrations must be measured on the same plate as the experimental sample since flavonoids demonstrate specific fluorescence behaviour and because in situ fluorodensitometry measurements are difficult to reproduce. The correlation between fluorescence and molecular structure is discussed briefly. The test conditions established are an improvement on the accepted in situ fluorodensitometry determination of flavonoids.

We also recommend Trading Suppliers and Manufacturers of 2-aminoethyl stearate (cas 10287-60-2). Pls Click Website Link as below: cas 10287-60-2 suppliers

Prev:Synthesis and structure of new tetracopper(II) complexes with N-benzoate-N′-[3-(diethylamino)propyl]oxamide as a bridging ligand: The influence of hydrophobicity on enhanced DNA/BSA-binding and anticancer activity
Next:The cobalt(II), nickel(II) and copper(II) complexes of 4-(2-aminoethyl)pyridine, p-phenetidine and N,N′-dimethylethanolamine and a study of their performance as colouring materials for poly(vinyl chloride))