Near-infrared fluorimetric determination of nucleic acids by shifting the ion-association equilibrium between heptamethylene cyanine and ALCIAN BLUE 8GX (cas 12040-44-7)

Add time:07/28/2019    Source:sciencedirect.com

A new method based on near-infrared (near-IR) fluorescence recovery, employing a two-reagent system which is composed of an anionic heptamethylene cyanine (HMC) and a polycationic phthalocyanine dye, Alcian blue 8GX, is presented for the determination of nucleic acids. With a maximum excitation wavelength at 766 nm and a maximum emission wavelength at 796 nm, the fluorescence recovery is linear with the concentration of nucleic acids added. Factors including the acidity of the medium, the reaction time, the optimal ratio of the two reagents, as well as the influence of foreign substance were all investigated. Meanwhile, the mechanism of fluorescence recovery was also studied. Under the optimal conditions, the linear ranges of the calibration curves were 10–250 ng ml−1 for calf thymus DNA (CT DNA) and 10–200 ng ml−1 for yeast RNA. The detection limits were 6.8 ng ml−1 for CT DNA and 6.3 ng ml−1 for yeast RNA, respectively. The method has been applied to the analysis of practical samples and the recovery results were satisfactory.

We also recommend Trading Suppliers and Manufacturers of ALCIAN BLUE 8GX (cas 12040-44-7). Pls Click Website Link as below: cas 12040-44-7 suppliers

Prev:Peroxymonosulfate-based cleaning technology for metal oxide-coated ceramic ultrafiltration membrane polluted by ALCIAN BLUE 8GX (cas 12040-44-7) dye: Radical and non-radical oxidation cleaning mechanism
Next:Temporal variation and interaction of full size spectrum Alcian blue stainable materials and water quality parameters in a reservoir)