Hepatic metabolism of 3-oxoandrost-4-ene-17β-carboxylic acid in the adult rat: formation of carboxyl-linked glucuronides both in vivo and in vitro

Add time:07/31/2019    Source:sciencedirect.com

The hepatic metabolism of 3-oxoandrost-4-ene-17β-carboxylic acid (etienic acid), a probable acidic catabolite of deoxycorticosterone, was investigated using rats prepared with an external biliary fistula. Metabolic products were identified by GC-MS after hydrolysis with β-glucuronidase and by proton nuclear magnetic resonance after Chromatographic purification of protected glucuronides. About 80% of the injected dose was secreted into bile in 20 hours. Three fully reduced etianic acids (3α-hydroxy-5α-, 3β-hydroxy-5α-, 3α-hydroxy-5β-androstan-17β-carboxylic acids) were identified as were several of their di- and trihydroxylated congeners. Glucuronides of these reduced and/or hydroxylated metabolites constituted over half of the recovered dose, with carboxyl-linked glucuronides predominating over 3-hydroxyl-linked glucuronides. The mode of glucuronidation correlated well with the ability of liver microsomes to form the corresponding compounds in vitro from the set of four 3,5-diastereomeric etianic acids.

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