Identification and quantitation of acyl thioesters by thin-layer chromatography of hydroxamic acid derivatives

Add time:07/29/2019    Source:sciencedirect.com

The reaction between medium and long chain fatty acyl thioesters and neutral hydroxylamine results in the formation of acyl hydroxamic acids. Separation and identification of acyl hydroxamates is facilitated by conversion of these compounds to their acetyl derivatives. Thin-layer chromatoplate and solvent systems are described which permit the separation of the acetyl acyl hydroxamates on the basis of degree of unsaturation and on the basis of chain length (C6–C18).

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