Enantiomeric analysis of linalool in teas using headspace solid-phase microextraction with chiral gas chromatography

Add time:08/04/2019    Source:sciencedirect.com

Linalool is a major volatile component of tea aroma. It occurs naturally as R-(−)- and S-(+)-linalool enantiomers, which exhibit entirely different sensorial properties. Using headspace solid-phase microextraction with chiral gas chromatography, we quantitated R-(−)- and S-(+)-linalool in teas. Optimal extraction conditions were as follows: CAR–DVB–PDMS fiber, 60 min at 60 °C, and tea/water ratio of 1:6 (w(g)/v(mL)). We measured linalool levels in five different teas, in fresh leaves of 14 different cultivars, and in samples collected during processing of green and black teas. Enantiomeric distributions of linalool were significantly different among the different teas and among the different tea cultivars. R-(−)-Linalool and S-(+)-linalool reached maximum levels during the rolling of black tea, with the levels declining drastically during green tea processing. Although tea germplasm was an important factor in determining the enantiomeric distribution of linalool in tea products, the processing steps also had a large impact on linalool levels.

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