Effects of adenosine 3′,5′-cyclic monophosphate, forskolin, and cholera toxin on hormone production in human term placental cells

Add time:07/14/2019    Source:sciencedirect.com

Trophoblast cells from human term placenta in monolayer culture were used to investigate the role of adenosine 3′,5′-cyclic monophosphate in the production of human chorionic gonadotropin and estradiol-17β. The intracellular concentration of adenosine 3′,5′-cyclic monophosphate was elevated by (1) addition of an adenosine 3′,5′-cyclic monophosphate analogue, 8-bromoadenosine 3′,5′-cyclic monophosphate, (2) inhibition of the hydrolysis of G-GTP complex by cholera toxin, and (3) direct stimulation of adenylate cyclase with forskolin. Addition of 2 mmol/L of 8-bromoadenosine 3′,5′-cyclic monophosphate markedly increased the accumulation of human chorionic gonadotropin in the culture medium on days 2, 3, and 4 of treatment. Likewise, addition of cholera toxin (0.2 μg/ml) or forskolin (50 μmol/L) also enhanced human chorionic gonadotropin production. On the other hand, the production of estradiol-17β was significantly inhibited by 8-bromoadenosine 3′,5′-cyclic monophosphate, at the same time that human chorionic gonadotropin production was enhanced in the same experiments. These results further support a differential role of adenosine 3′,5′-cyclic monophosphate on human chorionic gonadotropin and estradiol-17β production in the human term placenta.

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