Lifetime-based photoconversion of EGFP as a tool for FLIM
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Add time:07/30/2019 Source:sciencedirect.com
BackgroundEGFP is a fluorescent tag extensively used in biological and biomedical research. Over the years many researches have gathered collections of cell lines bearing specific EGFP-tagged proteins. Despite its popularity some photochemical properties of EGFP remain undocumented and unused. We report on so far unexplored lifetime photoconversion of EGFP usable in FLIM.
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