Separation of biotin and its analogs by high-performance liquid chromatography: Convenient labeling for ultraviolet or fluorimetric detection
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Add time:08/04/2019 Source:sciencedirect.com
d-Biotin, dethiobiotin, d-biotin sulfoxides, and d-biotin sulfone are converted to uv-absorbing or fluorescent derivatives by reaction with ω-4-dibromoacetophenone or 4-bromomethyl methoxy coumarin. High-performance liquid chromatographic separation conditions of these esters are described using C18 bonded phase.
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