Identification of multiple peptides homologous to cockroach and cricket allatostatins in the stick insect Carausius morosus

Add time:08/04/2019    Source:sciencedirect.com

Eighteen peptides were isolated from brain extracts of the stick insect Carausius morosus. The peptides were purified in four steps by high-performance liquid chromatography, monitored by their ability to inhibit juvenile hormone biosynthesis by corpora allata of the cricket Gryllus bimaculatus in vitro, and chemically characterised by Edman degradation and mass spectrometry. We obtained complete primary-structure information for nine peptides, four of which belong to the peptide family characterised by a common C-terminal pentapeptide sequence –YXFGLamide. The remaining five belong to the W2W9amide peptide family, nonapeptides characterised by having the amino acid tryptophan in positions 2 and 9. The amino-acid sequence of two other peptides could not be completely resolved by means of Edman degradation; however, these peptides could be allocated to the –YXFGLamide and the W2W9amide family, respectively, by comparison of retention times, co-elution and mass spectrometry. Both classes of neuropeptides strongly inhibit juvenile hormone biosynthesis in crickets but show no inhibiting effect on the corpora allata of the stick insect.

We also recommend Trading Suppliers and Manufacturers of ALLATOSTATIN B2 (cas 134760-78-4). Pls Click Website Link as below: cas 134760-78-4 suppliers

Prev:Isolation and characterization of eight myoinhibiting peptides from the desert locust, Schistocerca gregaria: new members of the cockroach allatostatin family
Next:Molecular Cloning, Genomic Organization, and Expression of a B-Type (Cricket-Type) Allatostatin Preprohormone from Drosophila melanogaster)