Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii λ-carrageenan using carrabiose (cas 19253-99-7) oligosaccharides prepared by enzymatic hydrolysis

Add time:07/31/2019    Source:sciencedirect.com

λ-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora λ-carrageenase. The main digestion products were fractionated and analysed by 1H and 13C NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose (cas 19253-99-7) oligosaccharide series indicating that the λ-carrageenase cleaves the β-(1→4) glycosidic bonds. 1H and 13C NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the λ-carrageenan spectra. Besides the typical oligo-λ-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii λ-carrageenan.

We also recommend Trading Suppliers and Manufacturers of carrabiose (cas 19253-99-7). Pls Click Website Link as below: cas 19253-99-7 suppliers

Prev:Biotransformation of naringin and naringenin by cultured Eucalyptus perriniana cells
Next:Folate-functionalized nanoparticles for controlled ergosta-4,6,8(14),22-tetraen-3-one delivery)