Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions
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Add time:08/01/2019 Source:sciencedirect.com
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysul-fosuccinimide to such reactions can greatly enhance the yields obtained.
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