2′, 3′-Dideoxycytidine represses thymidine kinases 1 and 2 expression in T-lymphoid cells

Add time:08/09/2019    Source:sciencedirect.com

In vitro culture of H9 human lymphoid cells in the presence of 5.0 μM dideoxycytidine (ddC), for about 40–45 days, selected cells (H9-ddC cells), which were resistant to the drug and cross-resistant to AZT (zidovudine) and 5-fluoro-2′-deoxyuridine (FdUR). The major mechanism of cross-resistance to AZT and FdUR in these cells was low cellular activity of thymidine kinase (TK). To explore molecular mechanisms of the reduced TK activity in H9-ddC cells, the mRNA expression of TK1 and TK2 and western blot analysis of TK1 protein were performed. RT-PCR analysis revealed that in H9-ddC cells the expression of both TK1 and TK2 mRNA was reduced to 27.1% and 79.4%, respectively. The reduced TK1 gene expression was confirmed by an absence of a detectable TK1 protein band in western blot of H9-ddC cells. These results demonstrate that long-term treatment of H9 cells in the presence of ddC down-regulated TK1 and TK2 gene expression and reduced the expression and activity of TK in the resistant cells.

We also recommend Trading Suppliers and Manufacturers of 2',3'-dideoxycytidine monophosphate (cas 104086-75-1). Pls Click Website Link as below: cas 104086-75-1 suppliers

Prev:The in vitro and in vivo anti-retrovirus activity, and intracellular metabolism of 3′-azido-2′,3′-dideoxythymidine and 2′,3′-dideoxycytidine are highly dependent on the cell species
Next:Photo protection of RNA building blocks: Adenosine 5′-monophosphate, cytidine 5′-monophosphate and cytosine)