Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent

Add time:08/04/2019    Source:sciencedirect.com

By use of the neutral alkylating agent iodo [14C1] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including the two chromatographically distinct forms of HP1 and a hitherto undetected component HP4. On ion-exchange chromatography, HP2 and the two forms of HP1 may be isolated in sufficient purity for sequence analysis.

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