Characterization of a dammarenediol (cas 14351-29-2) synthase in Centella asiatica (L.) Urban

Add time:08/04/2019    Source:sciencedirect.com

To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative β-amyrin synthase [Plant Cell Rep, 24:304–311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol (cas 14351-29-2) standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C. asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons.

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