Response of leaf spot-sensitive and tolerant muskmelon (Cucumis melo L.) cells to the phytotoxin RORIDIN E (cas 16891-85-3)

Add time:08/03/2019    Source:sciencedirect.com

The response of muskmelon mesophyll and suspension cells to roridin E, a phytotoxin produced by the fungal pathogen Myrothecium roridum, was monitored by measuring changes in the fluorescence of cells stained with merocyanine 540, an optical probe for changes in transmenbrane electrical potential (PD). The fluorescence emission from leaf spot-sensitive and tolerant muskmelon mesophyll cells was not affected by roridin E. However, suspension cells from only the leaf spot-sensitive cultivar Iroquois exhibited a significant increase in fluorescence after 5 min of exposure to 10 μM roridin E. These results suggest that roridin E may be a good in vitro selective agent for muskmelon suspension cells and that fluorescence measurements may be useful in screening muskmelon germplasm for resistance to M. roridum.

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