Differential inactivation of transforming DNA in vitro and in vivo by 4-hydroxyaminoquinoline 1-oxide

Add time:08/04/2019    Source:sciencedirect.com

An Hcr− strain of Bacillus subtilis showed about 10-fold higher sensitivity to killing by 4-hydroxyaminoquinoline 1-oxide (4HAQO) than the parental Hcr+ strain, and the same differential sensitivity was observed between the same pair of strains after UV irradiation or 4-nitroquinoline 1-oxide (4NQO) treatment. DNA extracted from Hcr− cells treated with 4HAQO showed higher transforming activity when assayed on Hcr+ cells than on Hcr− cells in parallel to the case of DNA extracted from 4NQO-treated cells. However, DNA treated in vitro with 4HAQO in buffer solution showed the same transforming activity on both Hcr+ and Hcr− cells. These results indicate that 4HAQO damage induced in DNA in vivo but not in vitro is repairable by the excision-repair mechanism.

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